We narrowed to 2,439 results for: sds

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pGL3-TK-5UTR-BsmBI-Luciferase

Plasmid

#114670

Purpose

For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

Depositor

Lynne Postovit

Article

Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

Insert

LacZ

Use

Luciferase

Tags

for cloning 5' UTR-Luciferase fusions

Expression

Mammalian

Promoter

lac promoter

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G228A

Plasmid

#84926

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G228A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+8A)-IDR-mCherry-CRY2-NLS

Plasmid

#145262

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+8A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+8A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-MED1long-IDR-YFP-NLS

Plasmid

#145276

Purpose

Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR long (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT G250A

Plasmid

#84925

Purpose

luciferase reporter for mutant TERT promoter

Depositor

Joseph Costello

Article

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

G250A

Promoter

TERT

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only
HA-Rab8a-WT

Plasmid

#101048

Purpose

Expresses HA-tagged human Rab8-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pSMPUW-miR-124-GFP-Puro

Plasmid

#117321

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Lentiviral

Tags

GFP-Puro

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145270

Purpose

Mammalian Expression of Nuclear Opto TBP(53Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(53Q)-IDR (TBP Human)

Expression

Mammalian

Mutation

Q-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-INPP5E-CAAX

Plasmid

#202746

Purpose

express plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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hum alpha ENAC promoter a25-a23

Plasmid

#83427

Purpose

promoter reporter construct to test 5' flanking sequences

Depositor

Christie Thomas

Article

Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

Insert

human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)

Use

Luciferase

Tags

Luciferase

Promoter

alpha ENAC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145261

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+7A)-IDR-mCherry

Plasmid

#145281

Purpose

Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)

Plasmid

#119315

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 366-852

Promoter

CMV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-mCherry-CRY2-NLS

Plasmid

#145260

Purpose

Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+10A)-IDR-mCherry

Plasmid

#145282

Purpose

Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+10A)-IDR (HOXD13 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-WT

Plasmid

#215601

Purpose

For overexpression of Gal4-DBD CEBPa WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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