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pGGG002

Plasmid

#48851

Purpose

Provides an H-A adapter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

H-A adapter - short

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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sgAAVS1(A)

Plasmid

#85570

Purpose

Expresses sgRNA targeting human AAVS1 locus

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Luc-Pten-3'UTR

Plasmid

#64098

Purpose

Pten Luciferase reporter

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

Pten 3'UTR (PTEN Human)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only
pSR-puro-Exo84

Plasmid

#31116

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

Exo84 shRNA (EXOC8 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only
pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-HypaCas9

Plasmid

#190604

Purpose

PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pExchange_tdk_BT1311p-tetR-2

Plasmid

#90326

Purpose

Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

TetR

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.Z

Plasmid

#79470

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Zeocin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-GFP

Plasmid

#27245

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

GFP

Use

Lentiviral

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pHiUGE HA-epitope donor ORF1

Plasmid

#200372

Purpose

Tagging endogenously expressed proteins with HA epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

HA epitope

Expression

Mammalian

Mutation

NA

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pP2-P1(S')

Plasmid

#196334

Purpose

Plasmid expressing chimeric P2-P1(S') tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-P1(S') and chaperone protein U'

Expression

Bacterial

Promoter

P2 late promoter

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO1811_Cas9_noNLS_human

Plasmid

#74490

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Expression

Mammalian

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-eGFP

Plasmid

#24557

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

eGFP

Use

Cre/Lox and Mouse Targeting

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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Endoplasmic reticulum-targeted optodPLD

Plasmid

#140059

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

Plasmid

#112840

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Full length Grb2

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Src(WT)-mCherry

Plasmid

#87356

Purpose

PI(WT): Photo-inhibitable/blue light sensitive ; WT: wild type

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-PAX9

Plasmid

#75079

Purpose

retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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TAL3179

Plasmid

#41353

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox2-Right (sox2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pSR-puro-RalBP1

Plasmid

#31115

Depositor

Christopher Counter

Article

Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

Insert

RalBP1 shRNA (RALBP1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only