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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,769 results for: puro

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Showing: 6601 - 6620 of 18769 results

  5. PB-PEmax NG

    Plasmid
    #187894
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, NG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187894-plasmid-map-sequence-id-370740

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  11. BARBEKO sgRNA library

    Pooled library
    #174163
    Purpose
    Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons.
    Depositor
    Wensheng Wei
    Publication
    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. dCas9-ddMSK1

    Plasmid
    #165603
    Purpose
    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)
    Depositor
    Isaac Hilton
    Publication
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 Human, S. Pyogenes, Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165603-plasmid-map-sequence-id-327022

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  14. pMH-EBFP

    Plasmid
    #161714
    Purpose
    Gateway-compatible destination vector for mammalian expression of N-terminal EBFP protein under CMV promoter
    Depositor
    Michael Huen
    Type
    Empty backbone
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    Jan. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    161714-plasmid-map-sequence-id-319502

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  17. AAVS1-PDL1

    Plasmid
    #159280
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d
    Insert
    Human PDL1/CD274 (CD274 Human)
    Use
    AAV; S1 knockin donor vector
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159280-plasmid-map-sequence-id-312917

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Showing: 6601 - 6620 of 18769 results