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We narrowed to 10,311 results for: otos

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Showing: 9931 - 9960 of 10311 results

  5. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  6. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  12. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid
    #121819
    Purpose
    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121819-plasmid-map-sequence-id-237368

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  19. Ce iPGM-C-HiBiT

    Plasmid
    #207120
    Purpose
    Bacterially expressed iPGM-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    C. elegans-iPGM HiBIT His10 (ipgm-1 Nematode)
    Tags
    HiBiT Tag and Thrombin cleavage site + 10x His Ta…
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207120-plasmid-map-sequence-id-417169

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  20. E. coli Lig-N-HiBiT

    Plasmid
    #215437
    Purpose
    Bacterially expressed E. coli Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], N-HiBiT, N-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215437-plasmid-map-sequence-id-428719

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  21. E. coli Lig-C-HiBiT

    Plasmid
    #215438
    Purpose
    Bacterially expressed E. coli Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    NAD-dependent DNA ligase LigA [Escherichia coli], C-HiBiT, C-10XHis.Thrombin
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215438-plasmid-map-sequence-id-428720

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  22. MAS1-DuET

    Plasmid
    #213335
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    MAS1 (MAS1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213335-plasmid-map-sequence-id-430866

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  26. pcDNA3.1 NKX6-2(1-196)-Venus

    Plasmid
    #209182
    Purpose
    Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated mutation containing the amino acids 1 to 196; fused with the green fluorescent protein Venus allowing the use of fluorescent based protocols.
    Depositor
    Federico Herrera
    Article
    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023
    Insert
    NKX6-2 (1-196) fused with Venus (NKX6-2 Human)
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209182-plasmid-map-sequence-id-413578

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  27. Cav2.2 BBS W391A pcDNA3

    Plasmid
    #206077
    Purpose
    Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding
    Depositor
    Annette Dolphin
    Article
    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    W391A
    Promoter
    CMV
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206077-plasmid-map-sequence-id-406768

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  29. GFP-Tac-STC

    Plasmid
    #162490
    Purpose
    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162490-plasmid-map-sequence-id-323468

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  30. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29 and 117-160 Bactrocera parkin(C463A)

    Plasmid
    #128383
    Purpose
    expresses protein UbcH7 (C86K) fused to Bactrocera parkin (C463A) with residues 1-29 and 117-160 deleted
    Depositor
    Kalle Gehring
    Article
    Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-
    Insert
    UbcH7 (UBE2L3 Human)
    Tags
    Bactrocera dorsalis parkin with mutation C463A an…
    Expression
    Bacterial
    Mutation
    mutation C86K in UbcH7 gene
    Promoter
    lac promotor
    Available Since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128383-plasmid-map-sequence-id-254044

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Showing: 9931 - 9960 of 10311 results