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pIS1-Vim/Actb5UTR-renilla

Plasmid

#38238

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Actb promoter (1000 nt upstream) and 5' UTR …

Promoter

Endogenous Vim

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
GFP Snail 8SA

Plasmid

#16229

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
UbC-blasticidine-P2A-TETon-3G transactivator

Plasmid

#200191

Purpose

A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

Depositor

Jacco van Rheenen

Article

Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

Insert

blasticidine - P2A - TETon-3G transactivator

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

UbC

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPqcxip-hSURF4-FLAG

Plasmid

#159139

Purpose

Hu SURF4 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

surfeit 4 (SURF4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-myr-CSK-GFP

Plasmid

#83470

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted-CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

doxycycline-inducible promoter

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter CREB site mutant pGL3Basic

Plasmid

#32732

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-51 CREB site TAACGTCA deletion

Promoter

-962 CCND1 Promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCRISPaint-mNeon-PuroR [M1G]

Plasmid

#171800

Purpose

Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mNeonGreen-T2A-PuroR

Use

Gene tagging

Mutation

PuroR: Changed Methionin 1 to Glycine.

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pclbw-PAGFPomp25

Plasmid

#58427

Purpose

Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

paGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

PAGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

cmv-bactin

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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ATF4 10: 5’ATF4.uORF1.GFP

Plasmid

#21857

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF1 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP

Plasmid

#198389

Purpose

GCN1 R2312A expresson

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

R2312A mutation in RWDBD (RWD binding domain)

Promoter

CMV

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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AT-U1

Plasmid

#112059

Purpose

U1 snRNA tagged with 1 copy of Riboglow (truncated variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

U1 (RNU1-1 Human)

Expression

Mammalian

Mutation

1 copy of Riboglow tag (truncated variant A) near…

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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