We narrowed to 18,241 results for: multi
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Plasmid#73035PurposeExpress an NT-3-BDNF hybrid neurotrophic factor in mammalian cellsDepositorInsertNT-3/D15A (NTF3 Human)
UseLentiviralExpressionMammalianMutationMutated Aspartic acid in position 15 of the matur…Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRJPaph-lacZYA
Plasmid#67037PurposePlasmid for Paph-lacZYA integration downstream of B. diazoefficiens (B. japonicum) scoIDepositorInsertsB. diazoefficiens DNA from scoI downstream region (comprising blr1132')
lacZYA
UseConjugative bacterial vectorAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
ExpressionMammalianPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050, Set A
Pooled library#104090PurposeCrispriDepositorUseLentiviralAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tre-Tight ifgMosaic Donor vector (LH121)
Plasmid#99630PurposeTo clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/LoxMutation(Please see depositor comments below)Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP
Plasmid#201681PurposeLentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expressionDepositorInsertsUseLentiviralExpressionMammalianAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-RKIP_RFP
Plasmid#199215PurposeRKIP (PEBP1) expression plasmid with a RFP reporter on the backbone.DepositorAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax SpG
Plasmid#187895PurposeAll-in-one prime editor piggyBac transposon with PEmax, SpG variantDepositorInsertSpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt(R23/25A)
Plasmid#181835PurposePhospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.DepositorInsertLyn-dInPAkt
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationArginines 23 and 25 in PH domain mutated to alani…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD4
Plasmid#173852PurposeProtein expression plasmid for recombinant His-Myc tag human PLD4DepositorInsertPLD4 (PLD4 Human)
Tags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_TPV-CXCL9-mCherry-SBP
Plasmid#170677PurposeExpresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.DepositorInsertsTagsSBP and mCherryExpressionMammalianPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-BlastR [M1G]
Plasmid#171814PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]DepositorInsertmScarlet-T2A-BlastR
UseGene taggingMutationBlastR: Changed Methionin 1 to Glycine.Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]
Plasmid#78174PurposeAAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterhuman synapsinAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACT1:Cas9-GFP, U6:sgUPRT
Plasmid#122853PurposeExpresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT geneDepositorInsertsCas9-GFP
U6-sgUPRT
UseCRISPR; Cas9-gfp plasmid for genome editing of cr…TagseGFPPromoterCryptosporidium parvum U6 and Cryptosporidium par…Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-T2A-moxMaple3
Plasmid#120875PurposeMammalian expression of mCherry and moxMaple3DepositorInsertsmCherry
moxMaple3
ExpressionMammalianMutationfrom mMaple3, moxMaple3 was engineered by replaci…Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Anti-GABA(B)R2 [N81/37.1R]
Plasmid#114544PurposeMammalian Expression Plasmid of anti-GABA(B)R2 (Human). Derived from hybridoma N81/37.1.DepositorInsertanti-GABA(B)R2 (Homo sapiens) recombinant mouse monoclonal antibody (GABBR2 Mouse)
ExpressionMammalianPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
ExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-T2A-mCherry-PTS1
Plasmid#87827PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
TagsEGFP and mCherryExpressionMammalianPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only