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pmRE-Tn5-142

Plasmid

#118531

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_15278a

Plasmid

#173545

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_15278_P17777

Expression

Plant

Promoter

35S

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1-287)

Plasmid

#182839

Purpose

CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

CBP1-287

Expression

Bacterial

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsC2orf68-EBMmut_K

Plasmid

#146761

Purpose

Mammalian Expression of HsC2orf68-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsC2orf68-EBMmut (C2orf68 Human)

Expression

Mammalian

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDON207 NSP3 K977Q

Plasmid

#180057

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 K977Q (ORF1ab SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 K977Q

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON207 NSP3 A890D

Plasmid

#180069

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP3 A890D (ORF1ab SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP3 A890D

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON221 ORF3a Q57H

Plasmid

#180072

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 ORF3a Q57H (ORF3a SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

ORF3a Q57H

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 N S235F

Plasmid

#180071

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 N S235F (N SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

N S235F

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 NSP5 K90R

Plasmid

#180073

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 NSP5 K90R (ORF1ab SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

NSP5 K90R

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.U6gT28.3.hSyn.H2B.RFP

Plasmid

#170370

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS11B

Plasmid

#172421

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 11B (TMPRSS11B Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 MYC dBR

Plasmid

#176046

Purpose

Expresses Myc Mutant (delta basic region) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 355-369

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-HE-bPAC(R278A)-Myc-eYFP

Plasmid

#165488

Purpose

Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

bPAC(R278A)-Myc-eYFP

Use

Cdna expression, xenopus oocyte

Tags

EYFP and Myc

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.H2B.RFP

Plasmid

#170372

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.4.EFS-NS.H2B-RFP

Plasmid

#170365

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.3.EFS-NS.H2B-RFP

Plasmid

#170364

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_14371

Plasmid

#173567

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_14371_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL86977OD:PITG_11947

Plasmid

#173566

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_11947_T30-4

Expression

Plant

Promoter

35S

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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