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S2_AscI_HA-gateway_Bsu36I

Plasmid

#128468

Purpose

destination vector with N-terminal HA tag

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

destination vector with N-terminal HA tag

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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2ERT2-AAVS1 TALEN-L

Plasmid

#120544

Purpose

Drug inducible TALEN targeting the human AAVS1 locus for genome editing

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

ERT2, hAAVS1 1L TALEN

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ePsuACR-mRuby2-ST

Plasmid

#109133

Purpose

Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ePsuACR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eiC++-mRuby2-ST

Plasmid

#109132

Purpose

Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eiC++-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-CsChR-mRuby2-ST

Plasmid

#109130

Purpose

Cation channelrhodopsin CsChR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

CsChR-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET15T+1X

Plasmid

#108188

Purpose

PCNA1-PdX fusion protein

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PCNA1-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ121

Plasmid

#99325

Purpose

Express two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Insert

TALE-VP64-T2A-TALE-VP64

Use

TALEN

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

Plasmid

#104615

Purpose

Expresses GFP upon binding of TALE4 to VAS4

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJC-20xUAS-TALE-VP64-P10

Plasmid

#104605

Purpose

Control plasmid (no TALE array) with P10 terminator

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Type

Empty backbone

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-61

Plasmid

#92262

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

NHA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-59

Plasmid

#92261

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Mutation

aa 940-1215

Promoter

Ac5

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProUBQ10 GW

Plasmid

#79751

Purpose

binary DEST vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Type

Empty backbone

Expression

Plant

Promoter

ProUBQ10

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CMV.Pro

Plasmid

#79486

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CMV Promoter

Use

Multisite gateway entry clone

Promoter

CMV Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

Plasmid

#87572

Purpose

PV4 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

Plasmid

#89056

Purpose

PV3 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBRN168-MXMT

Plasmid

#79819

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

RN168/DsREDmonomer (1–168)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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