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We narrowed to 47,358 results for: cha;

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Showing: 6661 - 6680 of 47358 results

  5. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Article
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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  14. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

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  15. kanMX6-ins2

    Plasmid
    #195039
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    KanR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195039-plasmid-map-sequence-id-384242

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  16. pOPINB-hRNF216(457-784)

    Plasmid
    #171922
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171922-plasmid-map-sequence-id-346327

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  17. pC-SUR-YR

    Plasmid
    #61768
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    Magnaporthe oryzae ILV promoter
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61768-plasmid-map-sequence-id-442790

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  18. p426_Cas9_gRNA-R-RDS1a

    Plasmid
    #87405
    Purpose
    p426_Cas9_gRNA-RDS1a without the ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting RDS1a sequence ATTCAATACGAAATGTGTGC in yeast chromosome 3.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting RDS1a
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87405-plasmid-map-sequence-id-193448

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  19. natMX6-ins5

    Plasmid
    #195042
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195042-plasmid-map-sequence-id-384245

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Showing: 6661 - 6680 of 47358 results