We narrowed to 125,204 results for: nin
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pSL21-VEX
Plasmid#158230PurposeRetroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)DepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianPromoterU6 promoter for crRNA expression and EFS promoter…Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-FURIN-puro
Plasmid#158460PurposeConstitutive lentiviral expression of human FURIN.DepositorAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM
Plasmid#45174DepositorTypeEmpty backboneTags6xHis and 6xHis/Thrombin/T7ExpressionBacterialPromoterT7Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3a-T1
Plasmid#41821PurposeExpresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineeringDepositorAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
Tagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
hfCas12Max
Plasmid#195336Purposevector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized hfCas12Max
Tags3xFlagExpressionMammalianMutationN243R, E336R, D892RPromoterCBh, CMV, hU6Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
TagsT2A iRFP670ExpressionMammalianAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta3
Plasmid#140988PurposeEncodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV-IFP2.0 T2A HO1
Plasmid#59427PurposeExpress infrared fluorescent protein IFP2.0 plus HO1 enzymeDepositorInsertIFP2.0T2AHO1 IRES EGFP
UseLentiviralPromoterCMVAvailable SinceDec. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ubi:Zebrabow
Plasmid#118219PurposeFor ubiquitous expression of Brainbow in zebrafishDepositorInsertBrainbow 1.0 L
UseZebrafish expressionAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP2
Plasmid#141256PurposeGateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)
Plasmid#185910PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9=D10APromoterCMV and T7Available SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hACE2-hygro
Plasmid#161758PurposeExpresses human ACE2 in mammalian cellsDepositorAvailable SinceNov. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIX403_UBAP2L_mCherry
Plasmid#105286Purposepuro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion proteinDepositorInsertUBAP2L (UBAP2L Human)
UseLentiviralTagsmCherryExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MH6-RspCas13d_RspCasWYL1
Plasmid#108304PurposeExpresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.DepositorInsertsRspCas13d
RspWYL1
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-CyOFP1
Plasmid#105799PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-CyOFP1ExpressionMammalianAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-RRvT
Plasmid#87364PurposeExpresses RRvT, a tandem heterodimer red fluorescent proteinDepositorInsertRRvT
TagsHis-TagExpressionBacterialPromoterpBad (Arabinose)Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TET-mSox2
Plasmid#20908PurposepiggyBac vector with tet inducible mSox2DepositorAvailable SinceApril 23, 2009AvailabilityAcademic Institutions and Nonprofits only
