Addgene

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pDONR223-JAK2

Plasmid

#23915

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK2 (JAK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GCaMP6f (AAV9)

Viral Prep

#135632-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Promoter

Tags

None

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
pBBR15.2-2H12.D11opt-scarRef

Plasmid

#221150

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2.1
mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2.1)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pORF1-Flag

Plasmid

#190565

Purpose

Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.

Depositor

Anthony Furano

Article

Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

ORF1 (from L1.3)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLifeAct-HyPer7

Plasmid

#136464

Purpose

Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Use

Tags

LifeAct

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Use

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE28

Plasmid

#90370

Purpose

TFEB - Lysosomal biogenesis gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TFEB

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PB-U6insert-EF1puro

Plasmid

#104537

Purpose

A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-DjCas13d-msfGFP-Blast

Plasmid

#226008

Purpose

DjCas13d protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

DjCas13d RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Use

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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MDM2p-Mdm2-YFP

Plasmid

#53962

Purpose

Expresses YFP tagged Mdm2 from MDM2 promoter

Depositor

Galit Lahav, Uri Alon

Article

Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

Insert

MDM2 (MDM2 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

MDM2

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pET24b-BG1.3

Plasmid

#199437

Purpose

expresses BAmGreen1.3 in bacterial cells

Depositor

Andrew Woolley

Article

Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

Insert

BAMGreen1.3

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-OMP25

Plasmid

#141150

Purpose

Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

OMP25 (Synj2bp Rat)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

OMP25 c terminus from addgene plasmid #69598

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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E-cadherin-GFP

Plasmid

#28009

Depositor

Jennifer Stow

Article

Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-

Insert

E-cadherin (CDH1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco

Plasmid

#40200

Depositor

Patrick Moore

Article

Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

Insert

MCPyV LT codon optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TEF

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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