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p(U6b-BsaI-gRNA)

Plasmid

#65956

Purpose

Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Expression

Insect

Promoter

U6

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-SIRT2

Plasmid

#66144

Purpose

Expresses SIRT2 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

Insert

SIRT2 (Sirt2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

E28G

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
pSR1058

Plasmid

#208743

Purpose

Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PHbs

Use

Synthetic Biology

Expression

Bacterial

Promoter

PHbs

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shLuc

Plasmid

#122287

Purpose

Knockdown control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for Luc

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pINS-Blast

Plasmid

#31388

Depositor

Yegor Vassetzky

Article

Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

Insert

bsd

Use

Cre/Lox

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1mWT

Plasmid

#35032

Depositor

Sheila Stewart

Article

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1mWT

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-Lysosomes-20

Plasmid

#57397

Purpose

Localization: Lysosome Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

LAMP1

Tags

mEos2

Expression

Mammalian

Mutation

D50E

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
NK802

Plasmid

#166056

Purpose

Puromycin resistance and mCherry expression for Salpingoeca rosetta

Depositor

Nicole King

Article

Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

Insert

Pac puromycin resistance gene

Use

S. rosetta expression

Tags

mCherry

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL.hPGK.GP91

Plasmid

#30477

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

hPGK/GP91

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2-DRv7

Plasmid

#78627

Purpose

GESTALT V7 barcode for zebrafish

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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iDuet011a

Plasmid

#17628

Depositor

Linzhao Cheng

Article

Zhou et al Stem Cells. 2007 Mar . 25(3):779-89.

Insert

TetO7 - UBC promoter - GFP - PGK promoter - Hygro

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pTHSSe_51

Plasmid

#109249

Purpose

p15A BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Dendra2-VASP-5

Plasmid

#57744

Purpose

Localization: Actin/Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

VASP

Tags

Dendra2

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-Cx43-7

Plasmid

#57365

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Cx43

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG

Plasmid

#158538

Purpose

Bacterial expression of rhomboid protease HiGlpG

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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7851 pUG72-HSC82in

Plasmid

#10894

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

HSC82 (HSC82 Budding Yeast)

Use

Cre/Lox

Expression

Yeast

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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mEos2-Paxillin-22

Plasmid

#57409

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Paxillin

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits