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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,185 results for: ADA

Showing: 6701 - 6720 of 10185 results

  1. pKK-TEV-MBP

    Plasmid
    #105787
    Purpose
    Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-MBP
    Expression
    Mammalian
    Available Since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105787-plasmid-map-sequence-id-211379

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  2. pKK-TEV-mRuby3

    Plasmid
    #105797
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mRuby3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105797-plasmid-map-sequence-id-203388

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  3. pLV-TetOn-ND-Cdt1-mCherry

    Plasmid
    #193761
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    ND-Cdt1-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Mutation
    amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…
    Promoter
    TRE
    Available Since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193761-plasmid-map-sequence-id-380233

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  4. p-mCherry-C1-Phobos_CA

    Plasmid
    #98169
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Phobos_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…
    Promoter
    CMV (+enhancer)
    Available Since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98169-plasmid-map-sequence-id-182726

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  10. qTAG-N-Blast-ultraID-LMNB1

    Plasmid
    #207775
    Purpose
    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207775-plasmid-map-sequence-id-416196

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  11. pAAV-EFS-DIO-GRAB_CRFmut

    Plasmid
    #208663
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    AAV
    Promoter
    EFS
    Available Since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208663-plasmid-map-sequence-id-412146

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  16. pKK-TEV-mCherry

    Plasmid
    #105796
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105796-plasmid-map-sequence-id-203387

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  20. p-mCherry-C1-Aurora_CA

    Plasmid
    #98170
    Purpose
    slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Peter Hegemann
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    CMV (+enhancer)
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    98170-plasmid-map-sequence-id-182711

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Showing: 6701 - 6720 of 10185 results