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pHH0103_DLG4-1/1

Plasmid

#91266

Purpose

Protein expression and purification of human SH3 domain construct DLG4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG4-1/1 (DLG4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM4

Plasmid

#101642

Purpose

Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

KCyG4219

Tags

6XHis

Expression

Bacterial

Mutation

C-terminal residues (221-223) deletion. Ser220Val…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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HeFm1SpCas9

Plasmid

#92110

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASKIN2-1/1

Plasmid

#91261

Purpose

Protein expression and purification of human SH3 domain construct CASKIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN2-1/1 (CASKIN2 Human)

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-249 delta166-172

Plasmid

#53550

Purpose

mammalian expression of TPP1 OB-fold domain with 166-172 replaced with "GSSG"

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

contains aa 88-249, with aa 166-172 replaced with…

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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SNX9-PX (246-376)

Plasmid

#119090

Purpose

Bacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX9-PX (246-376) (SNX9 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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PI3KC2gamma-PX (1200-1310)

Plasmid

#119121

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2gamma-PX (1200-1310) (PIK3C2G Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pMG046 pETARA-CK1α-His

Plasmid

#154074

Purpose

Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CSNK1A1 (CSNK1A1 Human)

Tags

GST and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

Plasmid

#161574

Purpose

Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-SpCas9-HF1 (without sgRNA)

Plasmid

#92102

Purpose

Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

Cbh

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry

Plasmid

#35504

Purpose

AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2(C128S/D156A)-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

C128S and D156A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK5-1/1-IS1/2

Plasmid

#91303

Purpose

Protein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK5-1/1-IS1/2 (DOCK5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Synapto-pHluorin

Plasmid

#168510

Purpose

Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

Synaptophysin-pHluorin (Syp Mouse)

Tags

pHluorin

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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NA20 LN16

Plasmid

#101870

Purpose

Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

Depositor

Paul Clapham

Article

Peters et al J Virol. 2004 Jul;78(13):6915-26.

Insert

Env (env )

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only