We narrowed to 15,982 results for: LEA

1. H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

   Plasmid

   #182918

   Purpose

   Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

   Depositor

   Daniel Gerlich

   Article

   Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

   Insert

   pH2B (H2BC16P Human)
   

   Tags

   mNeonGreen-FRB(DmrC)

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-TetO(3G)-GCaMP6f-WPRE

   Plasmid

   #186205

   Purpose

   Express GCaMP6f under TetO(3G)

   Depositor

   Naoshige Uchida

   Article

   Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Promoter

   TetO(3G)

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX_HA-C7orf26v2

   Plasmid

   #178536

   Purpose

   Subcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v2 (INTS15 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT15541_dCBE-SuperFi-Cas9

   Plasmid

   #184371

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dCBE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15543_dABE-SuperFi-Cas9

   Plasmid

   #184373

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT15545_dABE8e-SuperFi-Cas9

   Plasmid

   #184375

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE8e-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX_HA-C7orf26v1

   Plasmid

   #178535

   Purpose

   Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backbone

   Depositor

   William Hahn

   Article

   Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

   Insert

   C7orf26v1 (INTS15 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-EGFP-C1-HsNot7-M141R_R

   Plasmid

   #147379

   Purpose

   Mammalian Expression of HsNot7-M141R

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   HsNot7-M141R (CNOT7 Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnEA-NvG-HsNot7_9-260_R

   Plasmid

   #147369

   Purpose

   Bacterial Expression of HsNot7_9-260

   Depositor

   Elisa Izaurralde

   Article

   Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

   Insert

   HsNot7_9-260 (CNOT7 Human)
   

   Expression

   Bacterial

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmCCR4_A

    Plasmid

    #145894

    Purpose

    Insect Expression of DmCCR4

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmCCR4 (twin Fly)
    

    Expression

    Insect

    Mutation

    The following variations comprises from our S2 ce…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV Tmx4-V5 C209A

    Plasmid

    #175140

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C209A

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Tmx4-V5 C211A

    Plasmid

    #175139

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C211A

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV Hsd11b1-V5

    Plasmid

    #175147

    Purpose

    Lentiviral expression of mouse Hsd11b1-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Hsd11b1 (Hsd11b1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV Tmx4-V5 C209A, C211A

    Plasmid

    #175141

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C209A, C211A

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEZT-GST-SLFN2-T1

    Plasmid

    #174320

    Purpose

    Expression of recombinant GST-SLFN2-R67A, K68A, N71A

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GST-SLFN2-R67A, K68A, N71A (Slfn2 Mouse)
    

    Use

    Bacmam, baculovirus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEZT-GST-SLFN2-T2

    Plasmid

    #174321

    Purpose

    Expression of recombinant GST-SLFN2-K242A, K249A, Q301A

    Depositor

    Bruce Beutler

    Article

    Yue et al Science. 2021 May 14;372(6543). pii: 372

    Insert

    GST-SLFN2-K242A, K249A, Q301A (Slfn2 Mouse)
    

    Use

    Bacmam, baculovirus

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPodA11

    Plasmid

    #162843

    Purpose

    Expression protein designed PodA10 from a TEV cleavable IPTG inducible plasmid with ampicillin resistance

    Depositor

    Dianne Newman

    Article

    VanDrisse et al Proc Natl Acad Sci U S A. 2021 Mar 23;11

    Insert

    Protein Designed Pyocyanin demethylase
    

    Tags

    TEV-cleavable 6x-His tag

    Expression

    Bacterial

    Mutation

    A53N, I73T, A87V, M99V, A129T from WT PodA

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3HA-AMOT p130∆PPxY1(L106P)

    Plasmid

    #166714

    Purpose

    expresses AMOT(L106P) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 L106P (AMOT Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed leucine 106 to proline

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3HA-AMOT p130∆PPxY1(A112K)

    Plasmid

    #166715

    Purpose

    expresses AMOT(A112K) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 A112K (AMOT Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed alanine 112 to lysine

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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