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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Mor2 shRNA

Plasmid

#59298

Purpose

3rd generation lentiviral vector expressing shRNA to mouse Opmr1

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Mor2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
GWF303

Plasmid

#133611

Purpose

CD95-1-2xPP7, NLS-PCP-DNCR2-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CD95-1-2xPP7, PCP-DNCR2, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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GWF314

Plasmid

#133610

Purpose

CXCR4-com, NLS-com-GNCR1-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

CXCR4-6-com, com-GNCR1, BFP

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL712

Plasmid

#140382

Purpose

Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-otrO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pconst #2-RFP

Plasmid

#81076

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter- BBa_J23104

Available Since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0006

Plasmid

#42246

Purpose

gRNA targeted to zebrafish gene rgs4

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-rgs4 (rgs4 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA6-rbN1 S77A

Plasmid

#32945

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Change Serine77 to Alanine

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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Coho salmon GLUT4 (okGLUT4)

Plasmid

#65102

Purpose

Full length SLC2A4 cDNA (Oncorhynchus kisutch)

Depositor

Josep Planas

Article

Capilla et al Am J Physiol Endocrinol Metab. 2004 Aug;

Insert

SLC2A4

Expression

Bacterial

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
p-1459-adcy4-Luc

Plasmid

#28184

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

Adcy4 promoter (Adcy4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
TAL2381

Plasmid

#36790

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ABL1-TALEN_Right (Abl1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pGC4785

Plasmid

#113409

Purpose

Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

359_31121113_2

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSiP4

Plasmid

#21960

Depositor

Klaus Rajewsky

Article

Xiao et al Nat Immunol. 2008 Apr . 9(4):405-14.

Insert

Pten (Pten Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
CP1300-CBEmax

Plasmid

#119805

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1300) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-rbN1 S77D

Plasmid

#32947

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

slc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Serine 77 mutated to Aspartic Acid

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-N1-PDZ1-GAGA

Plasmid

#31651

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His, S-tag

Expression

Mammalian

Mutation

changed P(21)NGYGF changed to H(21)NGAGA

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-N1-PDZ2-GAGA

Plasmid

#32857

Depositor

Edward Weinman

Article

Weinman et al Biochemistry. 2003 Nov 4. 42(43):12662-8

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

His,S-tag

Expression

Mammalian

Mutation

PDZ2 P(161)NGYGF mutated to H(161)NGAGA

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-pnr

Plasmid

#71503

Purpose

flySTARR-seq screening vector with pnr core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1-spect-PthrC4-eGFP

Plasmid

#107410

Purpose

This plasmid contain promoter PthrC4, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.

Depositor

Kang Zhou

Article

Anilionyte et al Appl Microbiol Biotechnol. 2018 Aug;102(

Insert

eGFP

Expression

Bacterial

Promoter

PthrC4

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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