161,621 results

1. Cbh_v5 AAV-CBE C-terminal

   Plasmid

   #137176

   Purpose

   AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   v5 AAV-CBE C-terminal; U6-protospacer
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Neil Fairweather, Robert Fagan

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-ASAP5-Kv2.1-WPRE

   Plasmid

   #225707

   Purpose

   Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

   Depositor

   Michael Lin

   Article

   Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

   Insert

   ASAP5-Kv2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human synapsin

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Mutation

   Promoter

   CBh; U6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Xla.Tubb2:hArr-TEV;he1.1:eYFP

   Plasmid

   #218859

   Purpose

   Xla.Tubb2 promoter driving expression of human Arrestin-TEV protease fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hArr
   

   Use

   Tags

   human Arrestin-Tobbaco Etch Virus protease fusion…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ochre (rEcΔ2.ΔA.B3.tW*)

   Bacterial Strain

   #234622

   Purpose

   Genomically recoded bacteria for site-specific incorporation of two nonstandard amino acids into proteins

   Depositor

   Farren Isaacs

   Article

   Grome et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Bacterial Resistance

   Hygromycin

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CaMKIIa-hChR2(H134R)-EYFP (AAV5)

   Viral Prep

   #26969-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-EYFP (#26969). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-EYFP plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Promoter

   CaMKII

   Tags

   EYFP

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid

    #61628

    Purpose

    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    ntdTomato
    n2xEGFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    2 copies of EGFP with N-terminal SV40 NLS

    Promoter

    Auxin-dependent DR5 promoter containing 9 reverse…

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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