280,461 results

1. pLentiV2-dCas9-VP64

   Plasmid

   #141104

   Purpose

   Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

   Depositor

   Igor Ulitsky

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-HA-BACE1

   Plasmid

   #165032

   Purpose

   HA-BACE1

   Depositor

   Juan Bonifacino

   Article

   Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

   Insert

   BACE1 (BACE1 Human)
   

   Use

   Tags

   HA (embedded after propeptide cleavage site)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-HA-BACE1

   Plasmid

   #165032

   Purpose

   HA-BACE1

   Depositor

   Juan Bonifacino

   Article

   Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

   Insert

   BACE1 (BACE1 Human)
   

   Use

   Tags

   HA (embedded after propeptide cleavage site)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MCS-BioID2-HA

   Plasmid

   #74224

   Purpose

   To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   HA and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-DTR-GFP (AAV2)

   Viral Prep

   #124364-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-FLEX-DTR-GFP (#124364). In addition to the viral particles, you will also receive purified pAAV-FLEX-DTR-GFP plasmid DNA. CBA-driven, Cre-dependent expression of DTR-GFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Thomas Jessell, Eiman Azim

   Article

   Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

   Promoter

   Chicken B-actin

   Tags

   GFP (Cre-dependent)

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (AAV9)

   Viral Prep

   #124651-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (#124651). In addition to the viral particles, you will also receive purified pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 plasmid DNA. CamKIIa-driven expression of ChrimsonR fused to mScarlet fluorophore and targeted to neuronal soma for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Promoter

   CaMKII

   Tags

   mScarlet

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1 VDAC1-TC

    Plasmid

    #211733

    Purpose

    Expresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    Tetracysteine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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