We narrowed to 8,611 results for: mag

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDB-hCRY1-Halo-CD4-bla

Plasmid

#179445

Purpose

Donor vector to knock in Halotag C-terminal to human CRY1 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K1278R CRres si2,4R

Plasmid

#139406

Purpose

Dox-inducible, K1278R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Tags

6xHis

Expression

Mammalian

Mutation

K1278R

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-jYCaMP1

Plasmid

#135422

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEX-CFP-STIM1-deltaK

Plasmid

#18860

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Stop codon at amino acid position 671 of STIM1. C…

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-Rosmarinus

Plasmid

#183462

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

Rosmarinus

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mCarmine

Plasmid

#183464

Purpose

To produce AAV with the P3 promoter to express Alb-mCarmine fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mCarmine

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1

Plasmid

#133978

Purpose

mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Flag-RNF168 delta MIU1/MIU2

Plasmid

#133981

Purpose

mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1 and MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV TIP60 365-513

Plasmid

#78796

Purpose

To overexpress TIP60 365-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa365-513

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-6P-1-HRD1 aa 236-444

Plasmid

#185349

Purpose

RING domain-containing GST fusion protein for ubiquitination assays.

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

HRD1/SYVN (SYVN1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 236-444 only

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)3x

Plasmid

#112057

Purpose

ACTB mRNA tagged with 3 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

3 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)2x

Plasmid

#112056

Purpose

ACTB mRNA tagged with 2 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

2 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Halo-CD4-bla

Plasmid

#179451

Purpose

Donor vector to knock in Halotag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

HaloTag (Halo7 Synthetic)

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II tension sensor (FL-based)

Plasmid

#118721

Purpose

The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

inserted FL-based tension sensor module after aa1…

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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