-
Plasmid#59594PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP72-deltaFlincG
Plasmid#19733DepositorInsertdelta-FlincG (PRKG1 Bovine)
UseTagsHisExpressionBacterialMutationaa 77-356PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i2
Plasmid#59623PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-TTF2i1
Plasmid#59622PurposeExpression of shRNA against human TTF2DepositorInsertTTF2 (TTF2 Human)
UseRNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_4
Plasmid#60576Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_4
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spa)
Plasmid#133318Purposeexpresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pasteurianus)
UseUnspecifiedTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_2
Plasmid#60563PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_2
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSRP-mTAZ
Plasmid#31795DepositorInsertTAZ
UseRNAi and RetroviralTagsExpressionMutationPromoterRNA Pol-IIIAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-RNF40i2
Plasmid#59595PurposeExpression of shRNA against human RNF40DepositorInsertRNF40 (RNF40 Human)
UseRNAiTagsExpressionMammalianMutationPromoteruAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA
Plasmid#133342Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAXY0016
Plasmid#205585Purposep3flag_HygR(1-89)-NpuDnaE(N)DepositorInsertHygR-NpuDnaE(N)_homo-codon-OP
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReporter_7
Plasmid#60567PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_7
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTP130 (pTA106_TnsABC(Tn6900))
Plasmid#164261PurposeVector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.DepositorInserttnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
UseTagsExpressionBacterialMutation(tnsA) Changed Alanine 125 to Aspartic acidPromoterLactoseAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaCIDR
Plasmid#157872Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDRDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaCIDRPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Hmga2 m7
Plasmid#14792DepositorInsertHmga2 m7 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationHmga2 m7 mutant (see article and author's ma…PromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP
Plasmid#31478DepositorInsertSFFV HA NLS Y2 K227M T2A BFP
UseLentiviralTagsHA and NLSExpressionMutationF13Y, S111Y, K227M = Y2 nickase "reo" …PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pReporter_3
Plasmid#60564PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_3
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRTagsExpressionMutationPromoterxyloseAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_9
Plasmid#60569PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_9
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_4
Plasmid#60565PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_4
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pReporter_13_switchBP
Plasmid#62848PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.DepositorInsertReporter_13_switchBP
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1
Plasmid#133340Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentusDepositorInsertsgRNA_gcrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_11
Plasmid#60571PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_11
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBP-mTAZ-NFLAG
Plasmid#31791DepositorInsertTAZ (Wwtr1 )
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA
Plasmid#133334Purposefor constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pReporter_10
Plasmid#60570PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_10
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
A104T DJ-1 pET15b
Plasmid#60680PurposeRecombinant protein expression of Park7DepositorInsertA104T DJ-1 pET15b (PARK7 Human)
UseTags6xHisExpressionBacterialMutationA104TPromoterT7Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCVL SFFV Sbf-Sal linker
Plasmid#50434PurposeCloning vector for coexpression of gene cloned in between Sbf and Sal sites with BFP in mammalian cells. Made for cloning of meganuclease for direct comparison with megaTALs (pCVL TAL Xba-Sal).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMTG8-Amp-FRT
Plasmid#128277PurposeTIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFPDepositorInsertsTetR-LOV-TIP
EGFP
KRAS(G12V)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only