Addgene

We narrowed to 3,297 results for: aaas

Showing: 661 - 680 of 3297 results

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pAAV-AICD-NLS-IRES-hrGFP

Plasmid

#107543

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Localisation Signal (NLS: CCAAAAAAGAAGAGAAAGGTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NLS (APP Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAX APP Swe/Ind

Plasmid

#30145

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

human APP 695 Swedish/Indiana mutation (APP Human)

Use

Tags

Expression

Mammalian

Mutation

Swedish and Indiana; K595N, M596L and V642F

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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CD-MPR-GFP-RUSH

Plasmid

#202797

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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CD-MPR-mCherry-RUSH

Plasmid

#202798

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

M6PR (M6PR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Changed 40 K (AAA) with R (AGA)

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAX APP C99

Plasmid

#30146

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

APP C99 (APP Human)

Use

Tags

Expression

Mammalian

Mutation

C-terminal from BACE site of APP

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Aβ(M1-42/C18C33)

Plasmid

#173759

Purpose

Expresses the AβC18C33 peptide.

Depositor

James Nowick

Article

Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

Insert

Aβ(M1-42/C18C33) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

changed valine 18 to cysteine; changed glycine 33…

Promoter

T7

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJM656

Plasmid

#53086

Purpose

A non-Phosphatidylserine binding mutant form of Lactadherin(sGFP::LactC1C2mut) to be used as a control

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Use

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV-AICD

Plasmid

#69927

Purpose

bacterial expression of MBP-TEV-AICD

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Insert

AICD (APP Human)

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

47 aa long

Promoter

T7

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCAX APP delta CT

Plasmid

#30143

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

Expression

Mammalian

Mutation

deletion of C-terminal intracellular domain (afte…

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1–612

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pUCIDT-attL1-Human ABeta-attR5

Plasmid

#160436

Purpose

Entry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Homo sapiens amyloid beta precursor protein (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAX APPs-751-alpha

Plasmid

#30149

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Use

Tags

Expression

Mammalian

Mutation

splice variant 751; amino acids 1–612

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYI2294

Plasmid

#228352

Purpose

Methanol-inducible ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

KpAOX1 promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2165

Plasmid

#228347

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2216

Plasmid

#228349

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2292

Plasmid

#228350

Purpose

Methanol-inducible ALX-0171 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

KpAOX1 promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2293

Plasmid

#228351

Purpose

Constitutive ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

KpGAPDH promoter

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1461

Plasmid

#228319

Purpose

Doxycycline-regulatable ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Mutation

Promoter

Doxycycline-regulatable synthetic promoter (KpTc7…

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-CHST8 (3A)

Plasmid

#232651

Purpose

expresses CHST8 REP-to-AAA mutant in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CHST8 (3A) (CHST8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

R258A, E259A, P260A

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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