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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,441 results for: dcas9

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Showing: 661 - 680 of 2441 results

  4. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid
    #106895
    Purpose
    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106895-plasmid-map-sequence-id-201653

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  5. lenti-EF1a-dCas9-KRAB-MeCP2-Puro

    Plasmid
    #242163
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-puro
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242163-plasmid-map-sequence-id-496076

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  6. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

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  7. NLS-dCas9-HA-NLS-GFP4-VPR

    Plasmid
    #183924
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer
    Depositor
    Karsten Rippe
    Publication
    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-GFP4-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183924-plasmid-map-sequence-id-364146

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  8. pTRE3G-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192669
    Purpose
    Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192669-plasmid-map-sequence-id-379887

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  13. pEf1a-hifiDn29-dCas9-P2A-GFP

    Plasmid
    #247158
    Purpose
    Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hifiDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247158-plasmid-map-sequence-id-491688

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  14. VP64-dCas9

    Plasmid
    #177171
    Purpose
    VP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VP64-dCas9(D10A, H840A)
    Use
    CRISPR
    Tags
    NLS and NLS-3xFLag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177171-plasmid-map-sequence-id-348230

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  15. pGGB-OCS terminator-SunTag-HA-dCas9-NLS

    Plasmid
    #246798
    Purpose
    A GreenGate entry vector containing NLS-dCas9-HA-SunTag fused gene followed by a OCS terminator
    Depositor
    Danhua Jiang
    Insert
    NLS-dCas9-HA-SunTag-OCS terminator
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246798-plasmid-map-sequence-id-494307

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  16. PX856

    Plasmid
    #62888
    Purpose
    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa536-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS, NLS SV40, and VP64
    Expression
    Mammalian
    Mutation
    Asparagine 863 to Alanine (N863A)
    Promoter
    CBh
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62888-plasmid-map-sequence-id-105911

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  18. PX855

    Plasmid
    #62887
    Purpose
    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 (aa 2-535)
    Use
    CRISPR
    Tags
    FRB and NES PTK2
    Expression
    Mammalian
    Mutation
    Aspartic acide 10 to Alanine (D10A)
    Promoter
    CBh
    Available since
    March 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62887-plasmid-map-sequence-id-105910

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  19. LADL Anchor

    Plasmid
    #127664
    Purpose
    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter
    Depositor
    Jennifer Phillips-Cremins
    Publication
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CIBN-2A-Puro
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    Cas9 (D10A,H840A) mutant
    Promoter
    EF1a
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127664-plasmid-map-sequence-id-247037

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Showing: 661 - 680 of 2441 results