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Double UP mNeon to mScarlet FRT

Plasmid

#141111

Purpose

Natively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Depositor

Erik Dent

Publication

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNA-ccdB

Plasmid

#99915

Purpose

Basic cloning vector for the expression of imaging-based optimization sgRNA

Depositor

Yujie Sun

Publication

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHS_235

Plasmid

#195460

Purpose

Lentiviral Integratable, 4OHT/TMX-inducible expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - ZF3 - p65 - ERT2

Use

Lentiviral

Expression

Mammalian

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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SeAvs3

Plasmid

#188791

Purpose

Avs3 from Salmonella enterica NCTC13175

Depositor

Feng Zhang

Publication

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Expression

Bacterial

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Expression

Worm

Promoter

tag-168

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pET-21a_FnCpf1_2xNLS_protein_expression

Plasmid

#124548

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Publication

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnCpf1_2xNLS

Use

CRISPR

Expression

Bacterial

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah6

Plasmid

#122573

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1333

Plasmid

#119268

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1159

Plasmid

#119250

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.721

Plasmid

#108522

Purpose

PSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PSalTTC-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3 I0500 LasI

Plasmid

#107966

Purpose

Arabinose-inducible 3OC12HSL sender plasmid

Depositor

Jim Haseloff

Publication

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

lasI

Use

Synthetic Biology

Expression

Bacterial

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pBN260

Plasmid

#86722

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmyo-3::FLP D5

Expression

Worm

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBN278

Plasmid

#86724

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pmec-7::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAL-pgi_T

Plasmid

#74066

Purpose

pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

Depositor

Timothy Lu

Publication

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (T)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
TOPO HygroR

Plasmid

#68445

Purpose

TOPO construct expressing HygroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hygromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TOPO ZeoR

Plasmid

#68446

Purpose

TOPO construct expressing ZeoR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Zeocin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO PuroR

Plasmid

#68447

Purpose

TOPO construct expressing PuroR for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE60

Plasmid

#247036

Purpose

Lentiviral expression of a minimal promoter only with single TATA cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Available since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-synapsin-WHaloCaMP1a-EGFP

Plasmid

#205307

Purpose

Production of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoter

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a-EGFP

Use

AAV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAWP78-PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Plasmid

#198449

Purpose

Produces PVCs retargeted with expanded tropism hAd5 knob (RGD and PK7 insertions); also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5RGDPK7_Δpvc10

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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