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pAAV-BEC enhancer-minBG-Cre

Plasmid

#236724

Purpose

Cre Brain Endothelial Cell Enhancer

Depositor

Xiangmin Xu

Article

Velazquez-Rivera et al Neuron. 2025 May 21;113(10):1562-1578.e6

Inserts

cis-regulatory elements 89763
Cre Recombinase

Use

AAV

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA M3

Plasmid

#80936

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGK:TRL-EFS:iRFP670

Plasmid

#68475

Purpose

Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

tTR-KRAB-2A-rtTA3-2A-Luciferase
iRFP670

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EFS and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-ZETA

Plasmid

#53316

Purpose

Contains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

pds

Use

Low copy number bacterial cloning vector

Promoter

lazZ

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-mNINJ1-GFP

Plasmid

#208775

Purpose

Inducibly expresses mouse NINJ1 tagged with GFP

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

mNINJ1 (Ninj1 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ON VIPER CAR (anti-CD19)-final design

Plasmid

#192117

Purpose

Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)

Use

Lentiviral

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSBK.079

Plasmid

#187218

Purpose

Expresses Eco1 RT and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 Reverse Transcriptase
Cas1 + Cas2

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23115 and T7/lac

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-dzCas9-NG-Act3.0

Plasmid

#158415

Purpose

CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miRFP-6xFLAG

Plasmid

#161614

Purpose

Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

miRFP-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pemiRFP703-myosin

Plasmid

#136565

Purpose

LC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-myosin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-flex-Clover

Plasmid

#135177

Purpose

AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

Inserts

Clover
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR-NLS

Plasmid

#118467

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR-NLS

Tags

Nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA1-lenti

Plasmid

#115662

Purpose

Expresses AcrVA1 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA1

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pFPL-Yh2

Plasmid

#61650

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn-PdCO-mScarlet-WPRE (AAV5)

Viral Prep

#198510-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

Syn

Tags

mScarlet

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only