We narrowed to 7,159 results for: GFP expression plasmids
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Plasmid#25040DepositorInsertmiR-9 sponge
UseRetroviralTagsd2EGFPExpressionMammalianAvailable SinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQE81L-KOFP7
Plasmid#160330PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.DepositorInsertKOFP7
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-E
Plasmid#35978DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "E" HIV cladeAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-SunTag
Plasmid#158410PurposeCRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activationDepositorInsertdpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-D
Plasmid#35976DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "D" HIV cladeAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-C
Plasmid#35982DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "C" HIV cladeAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA-lib 2.0
Plasmid#89638PurposesgRNA expression in mammalian cells after Gateway cloningDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterhU6Available SinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
shNg Lenti FHRC3J1UGW
Plasmid#92233Purposelentiviral expression of EGFP and Ng shRNADepositorInsertshRNA targeting Ng
UseLentiviralExpressionMammalianPromoterH1Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT9750-BEAR-mCherry
Plasmid#162990PurposeBEAR target plasmid with split mCherry and disrupted 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-GCaMP6s
Plasmid#120896PurposeDonor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.DepositorInsertGCaMP6s
ExpressionMammalianPromoterCAGAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lck-mCherry-RGS
Plasmid#112935PurposePlasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)DepositorInsertp115RhoGEF-RGS domain
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0381
Plasmid#133231PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mMaple-OMP25
Plasmid#141151PurposePhotoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.DepositorInsertOMP25 (Synj2bp Rat)
TagsmMapleExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
ExpressionBacterialAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT-9222
Plasmid#124222PurposeCloning plasmid for EGxFP assay with self-targeting gRNA-sDepositorInsertself-targeting gRNA between EGFP halves
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
Plasmid#216170PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd
Plasmid#216172PurposeExpress the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- Control- ZEB2.locus
UseLentiviralTagsEGFPAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hMLH1dn-Hygro
Plasmid#195512PurposeLentiviral expression plasmid of hMLH1dn with P2A-HygroR markerDepositorInserthMLH1dn-P2A-HygroR (MLH1 Human)
UseLentiviralTagsP2AExpressionMammalianMutationHuman MLH1 del754-756PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only