We narrowed to 73,338 results for: LAS

1. pcDNA3β-FLAG-CBP-LD-HA

   Plasmid

   #32906

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   C/EBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   L1435A/D1436A

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

   Plasmid

   #165429

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII0.4

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDUAL_tacI

   Plasmid

   #155170

   Purpose

   BaSP and CuCBP co-expression: tacI promoter controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGTRm

   Plasmid

   #127197

   Purpose

   pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA PCR template
   

   Use

   Template plasmid for generation of golden gate pc…

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9675-CBE

   Plasmid

   #163007

   Purpose

   plasmid expressing CBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   CBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-sCAG-eGFP-ACAP1 F280E

   Plasmid

   #203801

   Purpose

   AAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   ACAP1 (ACAP1 Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   F280E

   Promoter

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP HDAC5 WT

   Plasmid

   #32211

   Depositor

   Reuben Shaw

   Article

   Mihaylova et al Cell. 2011 May 13;145(4):607-21.

   Insert

   HDAC5 (HDAC5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pAT9624-BEAR-cloning

    Plasmid

    #162986

    Purpose

    BEAR target cloning plasmid with split EGFP

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNAHygro(+)LAMP2AS

    Plasmid

    #86146

    Purpose

    Anti sense for LAMP2

    Depositor

    Janice Blum

    Article

    Zhou et al Immunity. 2005 May;22(5):571-81.

    Insert

    LAMP2a (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains first 525 bases of LAMP cDNA

    Promoter

    CMV

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDUAL_2_araBAD

    Plasmid

    #155176

    Purpose

    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG HDAC5 WT

    Plasmid

    #32213

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pV92.2

    Plasmid

    #78409

    Purpose

    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct

    Depositor

    Andrew Fire

    Article

    Silas et al Science. 2016 Feb 26;351(6276):aad4234.

    Inserts

    RT-Cas1
    Cas2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBA333

    Plasmid

    #214745

    Purpose

    Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT9658-sgRNA-mCherry

    Plasmid

    #162987

    Purpose

    sgRNA cloning plasmid

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    exchangeable cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-SYN-eGFP-Kindlin 1

    Plasmid

    #203733

    Purpose

    AAV vector plasmid expressing mouse kindlin-1 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    Fermt1 (Fermt1 Mouse)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-GGG-ELP(120nm)-Cys

    Plasmid

    #91572

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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