We narrowed to 15,981 results for: LEA

1. pCWXPG-UBI-Ngn3PDX1MafA

   Plasmid

   #114303

   Purpose

   Constitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptides

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Insert

   Ngn3 (NEUROG3 Human)
   

   Use

   Lentiviral

   Mutation

   Ngn3, PDX1 and MafA proteins are expressed from a…

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

   Plasmid

   #187584

   Purpose

   Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SP100 (SP100 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-actin-R62D

   Plasmid

   #118379

   Purpose

   Gateway compatible donor vector with actin-R62D.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   R62D

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDsRed-importinα

   Plasmid

   #119679

   Purpose

   Expresses DsRed-tagged human importinα in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   importinα (KPNA2 Human)
   

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   Contains amino acids 251-529

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HS-GFP-PACT

   Plasmid

   #105954

   Purpose

   heat shock inducible transgenesis, PACT is a centriol marker

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   PACT (AKAP9 Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pExodus CMV.Artemis

   Plasmid

   #40211

   Purpose

   Mammalian expression of codon-optimized Artemis.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Artemis (DCLRE1C Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

   Plasmid

   #187587

   Purpose

   Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SMN2 (SMN2 Human)
   

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-actin-V159N

   Plasmid

   #118383

   Purpose

   Gateway compatible donor vector with actin-V159N.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Mutation

   V159N

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP-CMV-GCaMP6-150

    Plasmid

    #91777

    Purpose

    Mammalian expression of low affinity calcium-sensing fluorescent protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

    Insert

    GCaMP6-150
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVH-EF1a-GFP-P2A-OCT4

    Plasmid

    #130692

    Purpose

    Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80062

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. OCT4-eGFP-2A-Puro

    Plasmid

    #31939

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Inserts

    OCT4 (POU5F1 Human)
    eGFP (eGFP )
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. KAT14-pDEST10

    Plasmid

    #118382

    Purpose

    HIS-tagged insect cell expression vector with KAT14.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    KAT14 (KAT14 Human)
    

    Tags

    HIS

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pbZIP9:GFP-GUS

    Plasmid

    #172483

    Purpose

    GFP-GUS driven by the bZIP9 promoter

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    bZIP9 promoter (BZIP9 Mustard Weed)
    

    Tags

    GFP and GUS

    Expression

    Plant

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBJG1 T7-8xHis-OGT K842M

    Plasmid

    #154272

    Purpose

    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Lysine 842 changed to Methionine- bacterial codon…

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

    Plasmid

    #187578

    Purpose

    Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    WRAP53 (WRAP53 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. OCT4-eGFP-PGK-Puro

    Plasmid

    #31937

    Purpose

    Oct4 targeting donor vector

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-MCP-SRSF3-V5-mCherry

    Plasmid

    #235090

    Purpose

    SRSF3 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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