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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,947 results for: emb

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Showing: 6901 - 6920 of 11947 results

  3. PH-miRFP-FRB

    Plasmid
    #193568
    Purpose
    Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.
    Depositor
    Justin Taraska
    Publication
    Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PH domain from PLC delta1 (Plcd1 Rat)
    Tags
    FRB (T2098L) and miRFP703
    Expression
    Mammalian
    Mutation
    PH domain
    Promoter
    CMV
    Available since
    Feb. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193568-plasmid-map-sequence-id-380166

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  4. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Publication
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    Tags
    EGFP
    Expression
    Yeast
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  7. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid
    #169487
    Purpose
    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCMV-driven membrane localization
    Expression
    Mammalian
    Promoter
    PhCMV
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    169487-plasmid-map-sequence-id-364214

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  9. EGFP-uTEV1-MAVS-mTA

    Plasmid
    #170999
    Purpose
    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Publication
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[R537A]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[R537A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170999-plasmid-map-sequence-id-335667

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  10. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Publication
    Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

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  13. pscPP7(2xWT)-EGFP

    Plasmid
    #140261
    Purpose
    PP7CP-dependent translational repression
    Depositor
    Hirohide Saito
    Publication
    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Two PP7CP-binding sites embedded in scaffold
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    140261-plasmid-map-sequence-id-312932

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  14. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Publication
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

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  15. pDUAL_2_araBAD

    Plasmid
    #155176
    Purpose
    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Publication
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    155176-plasmid-map-sequence-id-310563

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  16. pBICI_2_strong

    Plasmid
    #155168
    Purpose
    see pBICI_strong: exchanged origin of replication (pBR322) and introduced bom site
    Depositor
    Bernd Nidetzky
    Publication
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155168-plasmid-map-sequence-id-310553

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  20. pAL942

    Plasmid
    #119721
    Purpose
    precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
    Tags
    none
    Expression
    Bacterial
    Mutation
    Alanine 248 to Threonine, Alanine 188 to Cysteine…
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119721-plasmid-map-sequence-id-232465

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Showing: 6901 - 6920 of 11947 results