We narrowed to 12,230 results for: nsf

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pAAV-CAG-miRFP713-P2A-GFP

Plasmid

#197163

Purpose

Expresses the protein of miRFP713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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V1-MESA-45F-Tev

Plasmid

#84501

Purpose

MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V1-MESA-45F-Tev

Use

AAV

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL

Plasmid

#218756

Purpose

Provides NOT intersectional (Cre NOT Flp) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL

Plasmid

#218755

Purpose

Provides NOT intersectional (Flp NOT Cre) expression of TVA-mCherry

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

TVA-mCherry

Use

AAV

Tags

mCherry

Promoter

hSyn

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTP924 pHAGE2 CMV GFP

Plasmid

#199392

Purpose

Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Promoter

U1a

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)

Plasmid

#187067

Purpose

Gp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

miniABEmaxNG-Gp41

Use

AAV and CRISPR

Expression

Mammalian

Promoter

MHP1

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA

Plasmid

#187068

Purpose

Gp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

Gp41-ABEmaxC2‐NG

Use

AAV

Expression

Mammalian

Promoter

MHP1

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Article

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pMADM-beta

Plasmid

#36891

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

Beta-Geo
thymidine kinase

Expression

Mammalian

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pJEP205-AAV-TRE3G-GFP-pA

Plasmid

#164789

Purpose

AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-myrSNAP

Plasmid

#60098

Purpose

Expresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditional

Depositor

Gregory Jefferis

Article

Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

Inserts

SNAP-tag
histone-GFP

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Promoter

CaMKII

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Galt1-sCGrx1p-HA

Plasmid

#230028

Purpose

Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)

Tags

HA

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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V2-MESA-35F-M-tTA

Plasmid

#84502

Purpose

MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA

Depositor

Joshua Leonard

Article

Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

Insert

V2-MESA-35F-M-tTA

Use

AAV

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-VEGF(Brolucizumab) PAGER(Gq)

Plasmid

#230014

Purpose

Expresses anti-VEGF PAGER(Gq) in mammalian cell

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-Brolucizumab-TEVcs-ALFA-hM1D

Use

AAV

Expression

Mammalian

Mutation

W442A on hM1D

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-mCherry(LaM6) PAGER(Gi)

Plasmid

#230012

Purpose

Expresses anti-mCherry PAGER(Gi) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM4D

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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