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pCAG-mRuby2

Plasmid

#89686

Purpose

pCAG promoter driving mRuby2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRL692

Plasmid

#70284

Purpose

Tn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942

Depositor

Peter Wolk

Article

Koksharova et al J Bacteriol. 2002 Oct;184(19):5524-8.

Insert

Transposon Tn5-692

Expression

Bacterial

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDW445

Plasmid

#8843

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

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pDSG319

Plasmid

#115596

Purpose

surface-displayed Ag3, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(synapto).cpsfGFP.miRFP670nano3

Plasmid

#214926

Purpose

Expresses presynaptic non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(synapto).cpsfGFP.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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Ddo_porcupine-1

Plasmid

#107631

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

porcupine-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Ddo_piwi-1

Plasmid

#107628

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

piwi-1

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pANCH2-Nat

Plasmid

#87249

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Expression

Yeast

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-24

Plasmid

#79939

Purpose

PH-Late1-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-24

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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REV human OCT4 TALEN

Plasmid

#52413

Purpose

TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN

Use

TALEN

Promoter

CAGGS

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
mSECFP/pcDNA3

Plasmid

#51951

Purpose

mammalian expression of mSECFP, a monomeric super enhanced cyan fluorescent protein

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

mSECFP

Expression

Mammalian

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCAT009

Plasmid

#171635

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-B2M
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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p CIneo-RL

Plasmid

#115366

Purpose

Expression vector to produce humanized Renilla luciferase

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-CrmA

Plasmid

#11831

Depositor

Guy Salvesen

Article

Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

Insert

CrmA (CrmA Cowpox virus)

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pSG22-pBAD-TetR

Plasmid

#102451

Purpose

Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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DL033:cFos::eGFP-iPAK4

Plasmid

#177883

Purpose

eGFP-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

cFos

Available Since

July 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term His8 GFP

Plasmid

#160680

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDA027

Plasmid

#131385

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A, D702Y

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-FRBx5-His

Plasmid

#103784

Purpose

expresses His-tagged iPOLYMER component in E. coli for protein purification

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

CFP-FRBx5-His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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WT-Pan

Plasmid

#99638

Purpose

EGFP-mCherry control reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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