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pcDNA5/FRT/TO_LAMP1_HaloTag7

Plasmid

#175531

Purpose

Lyzosomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPEPX-Plac

Plasmid

#122632

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCytERM-mNeptune2.5-N1

Plasmid

#197194

Purpose

Expresses the fusion protein of CytERM-mNeptune2.5 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-mNeptune2.5

Expression

Mammalian

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v1-P2A-sfGFP

Plasmid

#61057

Purpose

sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v1 and sfGFP

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
pBAD/HisD-LSSmKate2

Plasmid

#31909

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate2

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pPEPY-Plac

Plasmid

#122634

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJYP1

Plasmid

#202406

Purpose

Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

Depositor

Remy Loris

Article

Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

Type

Empty backbone

Expression

Bacterial

Promoter

fdeA

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-T4uvsW

Plasmid

#228201

Purpose

Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

Depositor

Stephen Benkovic

Article

Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

Insert

uvsW

Tags

hexahistidine and 10 amino acid linker

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Expression

Bacterial

Promoter

T7

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP682

Plasmid

#45454

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP682

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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GPI-mNeonGreen(2x)

Plasmid

#197913

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen-mNeonGreen

Expression

Mammalian

Mutation

none

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-BlastR

Plasmid

#207176

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD05

Plasmid

#208259

Purpose

Production of isopimara-8,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
sare_1287

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKSCTMF

Plasmid

#85073

Purpose

Gene insertion/deletion in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi

Insert

AOX1promoter - mazF - AOX1 tt- Zeocin R gene

Use

Gene insertion/deletion in pichia pastoris

Promoter

pAOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJDC182

Plasmid

#60854

Purpose

Expresses firefly luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Firefly luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-SNAPf

Plasmid

#188941

Purpose

T7 expression vector for making his6- and HA3-tagged SNAP protein

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-SNAPf

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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