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We narrowed to 6,241 results for: cas9 expression plasmid

Showing: 1361 - 1400 of 6241 results

  2. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  3. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  4. pJAT11

    Plasmid
    #204290
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204290-plasmid-map-sequence-id-405507

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  5. pJAT75

    Plasmid
    #204306
    Purpose
    Flippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204306-plasmid-map-sequence-id-406175

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  6. pJAT62

    Plasmid
    #204301
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204301-plasmid-map-sequence-id-405845

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  7. pJAT43

    Plasmid
    #204299
    Purpose
    For introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204299-plasmid-map-sequence-id-406188

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  8. pJAT56

    Plasmid
    #204300
    Purpose
    Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

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  9. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  10. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  11. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

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  12. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73196-plasmid-map-sequence-id-134659

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  14. pCRISPRyl

    Plasmid
    #70007
    Purpose
    CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al ACS Synth Biol. 2015 Dec 29.
    Inserts
    Codon optimized Cas9 from S. pyogenes
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70007-plasmid-map-sequence-id-137578

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  15. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Promoter
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  17. pJAT58

    Plasmid
    #204304
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204304-plasmid-map-sequence-id-406184

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  18. pJAT71

    Plasmid
    #204305
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204305-plasmid-map-sequence-id-405846

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  19. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Use
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

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  23. pBSE901

    Plasmid
    #91709
    Purpose
    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance
    Depositor
    Qi-Jun Chen
    Article
    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91709-plasmid-map-sequence-id-180109

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  24. pXSE901B

    Plasmid
    #91713
    Purpose
    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91713-plasmid-map-sequence-id-180059

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  27. pXSE901BG

    Plasmid
    #91714
    Purpose
    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance
    Depositor
    Qi-Jun Chen
    Article
    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91714-plasmid-map-sequence-id-180060

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  29. pRGEB33

    Plasmid
    #158151
    Purpose
    Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.
    Depositor
    Kabin Xie
    Article
    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    158151-plasmid-map-sequence-id-320624

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  30. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  32. BPK2101

    Plasmid
    #65770
    Purpose
    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    Use
    CRISPR
    Tags
    NLS-3xFlag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65770-plasmid-map-sequence-id-112650

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  33. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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  34. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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Showing: 1361 - 1400 of 6241 results