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pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pJK687
Plasmid#71640PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met mutant (TdPurE2-K41M)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methioninePromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-PIK3CA H1047R
Plasmid#180031PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsHA tagExpressionMammalianMutationH1047RPromoterEF1aAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK650
Plasmid#72400PurposeProduces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK650
Plasmid#72400PurposeProduces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
UseTagsExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHM_YEAST
Plasmid#204231PurposeBacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM_YEAST (MDH1 Budding Yeast)
UseTags6X HisExpressionBacterialMutationDoes not include transit peptide and includes a M…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXC6 (human) HIS-tag pET
Plasmid#8565DepositorInsertHOXC6 (HOXC6 Human)
UseTagsHis and T7ExpressionBacterialMutationThis protein starts at an internal Met initially …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHNP_ARATH
Plasmid#204238PurposeBacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHNP_ARATH (AT5G58330 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes not include the chloroplastic transit sequen…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT 6FLAG
Plasmid#182509PurposeC-SWAT compatible vector for C-terminal tagging with 6 FLAGDepositorInsert6 FLAG
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP
Plasmid#182510PurposeC-SWAT compatible vector for C-terminal tagging with GFPDepositorInsertGFP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HCMV UL97 KD
Plasmid#26688DepositorInsertUL97 (UL97 Human Cytomegalovirus (HHV-5))
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant Lys 355 mutated to MetPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-M1A(KaganF58)
Plasmid#52001PurposeExpresses the human MAVS CDS containing the point mutation M1ADepositorInsertMAVS
UseTagsExpressionMammalianMutationchanged Met 1 to AlaPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2B
Plasmid#105501PurposeExpression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression levelDepositorInsertdH2B
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
HHV6 U69 KD
Plasmid#26690DepositorInsertU69 (U69 Human Herpes Virus 6)
UseTagsHAExpressionMammalianMutationKinase Deficient Mutant: Lys 218 mutated to MetPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only
