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Plasmid#221186PurposeHis6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)DepositorInsertcdGreen
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET20b-NLuc-MBP-6xHis
Plasmid#135931PurposeExpresses NanoLuciferase in bacterial cells.DepositorInsertNanoLuciferase
UseTagsMBP-6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWXLd-RIEP-CeNLuc
Plasmid#135934PurposeExpresses the CeNLuc LumiFluor in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_hsPUM1-mCherry
Plasmid#107607PurposeTransient expression of Pumilio1, fused to mCherry in mammalian cells, under a hUBC promoterDepositorInserthsPUM1-mCherry
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterhuman UBCAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mut1-RS
Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-ACTU−
Plasmid#198869PurposeOptogenetic recruitment construct to increase inner membrane surface chargeDepositorInsertRad17 polyanionic tail (-14)
UseSynthetic BiologyTagsCRY2PHR-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE-EGFP_IRES-Blast
Plasmid#213113PurposeExpression of MinE-EGFP in mammalian cellsDepositorInsertMinE-EGFP (minE Escherichia coli)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1
Plasmid#214994PurposeExpress the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.DepositorInsertEEA1 (EEA1 Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
Plasmid#192636PurposeExpresses ERT2-CreN59-nMag-NLS with mCherry marker.DepositorInsertERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC hNLRP3-mNG
Plasmid#218642PurposeExpresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selectionDepositorInsertNLRP3 (NLRP3 Human)
UseLentiviralTagsmNeonGreenExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-EYFP-Kv
Plasmid#89256PurposeChannelrhodopsin localized to neuronal soma via a Kv2.1 targeting motifDepositorInsertChannelrhodopsin
UseAAVTagsEYFPExpressionMammalianMutationH134RPromotersynapsinAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-QFGal4-SV40pA
Plasmid#155118PurposeMiddle entry vector containing QFGal4DepositorInsertQFGal4
UseGateway middle entry vectorTagsExpressionMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE3-NLS
Plasmid#138218PurposeNuclear-targeted FRET-based cAMP indicator.DepositorInsertICUE3-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW533 (Peft-3::cGAL-N::let-858 3'UTR)
Plasmid#107132PurposecGAL-N driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHW531 (Peft-3::cGAL-C::let-858 3'UTR)
Plasmid#107136PurposecGAL-C driver under the control of C. elegans eft-3 promoterDepositorInsertNLS::gp41-1-C-intein::cGAL(AD)
UseTagsExpressionWormMutationPromoterC. elegans eft-3 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPIDNB2
Plasmid#169902PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.DepositorTypeEmpty backboneUseAvian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1aL-mCherry-W
Plasmid#126703PurposeConstitutive, ubiquitous mCherry expression.DepositorInsertmCherry
UseLentiviralTagsExpressionMutationPromoterEf1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LOV-Turbo-His6_pET21a
Plasmid#199668Purposeexpresses LOV-Turbo in bacteriaDepositorInsertLOV-Turbo
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only