We narrowed to 14,088 results for: ef1a;

1. Lv EF1a dCas9-HA 2A Blast

   Plasmid

   #102812

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   dCas9-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A (dCas9)

   Promoter

   EF1a

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 EF1a-EGFP

   Plasmid

   #231925

   Purpose

   3rd generation lentiviral transfer plasmid with EGFP reporter gene

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PEW-pEF1A-inactEGFP

   Plasmid

   #212009

   Purpose

   Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

   Depositor

   Catherine Wu

   Article

   Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

   Insert

   Inactivated eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-EF1aL-TagBFP-W

   Plasmid

   #126687

   Purpose

   Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV-EF1amCherry-2A-OsTIR1-W

   Plasmid

   #159296

   Purpose

   Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   OsTIR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

   Plasmid

   #32627

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Donor + Sce BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-FLEX-GT

   Plasmid

   #26198

   Depositor

   Edward Callaway

   Article

   Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   TVA mammalian codon optimized

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSicoR-Ef1a-mCh

   Plasmid

   #31847

   Purpose

   Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-vCre

   Plasmid

   #55638

   Purpose

   EF1a-driven expression of VCre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   vCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-V5-Bet1

    Plasmid

    #120243

    Purpose

    Expresses V5-tagged Bet1 in lentiviral vector

    Depositor

    Larry Gerace

    Insert

    Bet1 (Bet1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-iFlpV

    Plasmid

    #140137

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iFlpV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-REPORT(EF1a)

    Plasmid

    #172326

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_EF1a-->GssmCherry-PNE

    Plasmid

    #162157

    Purpose

    Lentiviral vector for constitutive expression of mCherry and PNE anchor in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-EF1a/b-actin

    Plasmid

    #82583

    Purpose

    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-oScarlet

    Plasmid

    #137135

    Purpose

    Viral expression of oScarlet

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_Ef1a:PE2-P2A-hMLH1dn

    Plasmid

    #184444

    Purpose

    Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

    Depositor

    Bobby Koeleman

    Article

    Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Ef1A

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-EF1aL-dsRed-W

    Plasmid

    #195863

    Purpose

    lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

    Depositor

    Darrell Kotton

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-EF1aGFP2AKAT7(E508Q)-W

    Plasmid

    #159293

    Purpose

    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

    Insert

    KAT7 E508Q
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

    Promoter

    human EF1a promoter

    Available since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-LIC-V5

    Plasmid

    #120248

    Purpose

    For LIC cloning with C-terminal V5 tag

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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