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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,850 results for: nin

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Showing: 681 - 700 of 66850 results

  14. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122650-plasmid-map-sequence-id-437776

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  16. pHelper_ShCAST_sgRNA

    Plasmid
    #127921
    Purpose
    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).
    Depositor
    Feng Zhang
    Publication
    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    Available since
    July 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127921-plasmid-map-sequence-id-333562

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  17. lentiGuide-Hygro-dTomato

    Plasmid
    #99376
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-dTomato
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99376-plasmid-map-sequence-id-191027

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  19. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70708-plasmid-map-sequence-id-364684

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  20. pcDNA3-10His-mStayGold (E138D)

    Plasmid
    #211363
    Purpose
    Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Publication
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mStayGold
    Tags
    10xHis-tag
    Expression
    Mammalian
    Mutation
    E138D
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    211363-plasmid-map-sequence-id-417674

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Showing: 681 - 700 of 66850 results