We narrowed to 3,199 results for: plasmid for e coli
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Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only
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pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialMutationPromoterpJ21306Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationE59G - please see depositor commentsPromoterAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
FruR-pHG165a (CRA)
Plasmid#90066Purposefull length WT FruR (CRA)DepositorInsertFruR (CRA)
UseTagsExpressionBacterialMutationPromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_mTq-Tat (c-plasmid)
Plasmid#127635PurposeEncodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.DepositorInsertmTurquoise2
UseSynthetic BiologyTagsRNA binding peptide: PCP and RNA binding peptide:…ExpressionBacterialMutationPromoterAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSP019
Plasmid#168195Purposeexpresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.DepositorInserteatA[H134R]
UseTagsExpressionBacterialMutationH134R mutationPromoteraraBADAvailable SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE502B
Plasmid#128971PurposeBroad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxyl-PmAvailable SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE302A
Plasmid#128970PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromotercI-pLAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNKR1
Plasmid#67968PurposeHigh copy number plasmid encoding part of the tra Operon PY-traRDepositorInserttra Operon (First Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNKR2
Plasmid#67969PurposeHigh copy number plasmid encoding part of the tra Operon traC-artADepositorInserttra Operon (second Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
UseTagsExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEL2200
Plasmid#170407PurposeGenetic complementation plasmid of LetA-[LetB PLL6->3] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 347-360)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2201
Plasmid#170408PurposeGenetic complementation plasmid of LetA-[LetB PLL6->5] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 580-600)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2254
Plasmid#170409PurposeGenetic complementation plasmid of LetA-[LetB PLL6->2] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 225-249)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2255
Plasmid#170410PurposeGenetic complementation plasmid of LetA-[LetB PLL6->4] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 445-479)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2256
Plasmid#170411PurposeGenetic complementation plasmid of LetA-[LetB PLL6->7] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 812-836)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2199
Plasmid#170406PurposeGenetic complementation plasmid of LetA-[LetB PLL6->1] mutantDepositorUseTagsExpressionBacterialMutationLetA-LetB(Δ702-717, replace with 114-130)PromoterAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_Cas9d_Plasmid
Plasmid#239445PurposeExpression plasmid codon-optimized for E. coliDepositorInsertCas9d
UseTagsExpressionBacterialMutationPromoterT7Available SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only