We narrowed to 11,558 results for: kars;

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pcDNA3-mRuby2-MACROD1_d77

Plasmid

#172588

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1del1-77

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

deleted amino acids 1-76

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713

Plasmid

#103818

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713 (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Mac 3.0-EYFP

Plasmid

#35515

Purpose

Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eMac 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking signal, ER export

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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paGFP-HRas G12V

Plasmid

#18694

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

H-Ras G12V (HRAS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCI-ING1a

Plasmid

#79069

Purpose

Expression of p47ING1 in mammalian cells

Depositor

Karl Riabowol

Article

Soliman et al Aging Cell. 2008 Dec;7(6):783-94. doi: 1

Insert

ING1 (p47 ING1A isoform) (ING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCold TDP43 G294A

Plasmid

#27452

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

Factor Xa and His6

Expression

Bacterial

Mutation

Glycine 294 mutated to Alanine (G294A)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pROF402

Plasmid

#155334

Purpose

Binary vector for expression of FLuc controlled by the blue light-repressible system

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAf-CRISPR-phoA

Plasmid

#207991

Purpose

CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

Insert

phoA

Use

CRISPR

Promoter

Aspergillus flavus U6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG S297A

Plasmid

#169949

Purpose

Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S297A

Promoter

CMV

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO 3xFlag-m-PKD1 S4134D-2xMyc/His

Plasmid

#83453

Purpose

Full-length murine PC1 with a Calmodulin binding mutation for Eukaryotic Expression (1037)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

polycystin-1 (Pkd1 Mouse)

Tags

2xMyc, 3xFlag, and 6xHis

Expression

Mammalian

Mutation

S4144D

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP

Plasmid

#103815

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero7

Plasmid

#187926

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero7

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero7 (SERF2 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-Myr-FLAG-p110α-DM

Plasmid

#55713

Purpose

eukaryotic expression of myristoylation tag (Myr-FLAG) p110a RBD double mutant T207D, K227A

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha (Pik3ca Mouse)

Tags

myristoylation tag (Myr-FLAG)

Expression

Mammalian

Mutation

T207D, K227A

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B CARD8 UPA-CARD-mCherry WT

Plasmid

#164011

Purpose

Mammalian expression vector encoding CARD8 UPA-CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pPHR-EYFP-NLS

Plasmid

#103820

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-NLS (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD1

Plasmid

#172584

Purpose

For transient expression of N-terminal GFP-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD2

Plasmid

#172585

Purpose

For transient expression of N-terminal GFP-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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cmv hTid Long

Plasmid

#13707

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Long (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
PHR-GFP

Plasmid

#183926

Purpose

Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Trib1

Plasmid

#131156

Purpose

Mammalian expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP

Plasmid

#103817

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP (CRY2 Mustard Weed)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

Plasmid

#172581

Purpose

For transient expression of C-terminal BirA-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

BirA(R118G)-HA

Expression

Mammalian

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-PKCsub(T/A)-YPet-NES

Plasmid

#138208

Purpose

Encodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKCsub(T/A)-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Sept. 26, 2021

Availability

Academic Institutions and Nonprofits only

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MigR1-Flag-TRIB1

Plasmid

#131158

Purpose

Retroviral expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MigR1-Myc-Nterm TRIB2

Plasmid

#131153

Purpose

Retroviral expression of N terminus of mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 1-145

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12-S35

Plasmid

#39511

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-G37

Plasmid

#39512

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and E37G

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only