We narrowed to 7,359 results for: GFP expression plasmids

1. pBRC169-MXMT

   Plasmid

   #79820

   Purpose

   DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Insert

   CaMXMT1
   

   Use

   Reporter plasmid

   Tags

   RC169/DsREDmonomer (169–225)

   Expression

   Plant

   Promoter

   CaMV 35S promoter and CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDL001_Hec1-ms_IgG_HC

   Plasmid

   #193618

   Purpose

   Hec1 heavy chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL002 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 Heavy Chain (Hec1-ms_IgG_HC)
   

   Tags

   His6 and signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDL002_Hec1-ms_IgG_LC

   Plasmid

   #193619

   Purpose

   Hec1 light chain variable and constant region (mouse) + exogenous N-term signal peptide. For co-expression with pDL001 in HEK293 cells.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-Hec1 light chain (Hec1-ms_IgG_LC)
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. FHp5pUP95C3,5SGW (E5)

   Plasmid

   #74035

   Purpose

   lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Mutation

   PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

   Promoter

   H1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

5. FUP95DSKGW (C8)

   Plasmid

   #74024

   Purpose

   lentiviral expression of Psd95-delta SK-EGFP fusion

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

   Insert

   Psd95 (DLG4 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   delta SK

   Promoter

   hUbC

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBFC1207

   Plasmid

   #186694

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_2xBsaI_NT (Guide Stuffer) crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Expression

   Bacterial

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUt-mNF-BACH1

   Plasmid

   #199219

   Purpose

   LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

   Depositor

   Gabor Balazsi

   Article

   Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

   Insert

   hTetR::P2A::EGFP::BACH1 (BACH1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV D2ir promoter

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

   Plasmid

   #225947

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S3E S17E S19E

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

   Plasmid

   #225948

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S3A S17A S19A

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid

    #225956

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Full-length CKAP4 with additional luminal domain …

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid

    #225944

    Purpose

    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    ATL1 (ATL1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid

    #119554

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRUPATH Triple Ga12

    Plasmid

    #196061

    Purpose

    Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlpha12-RLuc8 (GNA12 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRUPATH Triple Gai3

    Plasmid

    #196050

    Purpose

    Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai3-RLuc8 (GNAI3 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDL023_Tub-rb_IgG_HC

    Plasmid

    #193620

    Purpose

    α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Tubulin scFv heavy variable + rabbit constant chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDL024_Tub-rb_IgG_LC

    Plasmid

    #193621

    Purpose

    α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-Tubulin scFv light variable + rabbit constant chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV PGK1-3xHA

    Plasmid

    #220913

    Purpose

    Expresses PGK1-3xHA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-3xHA (PGK1 Human)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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