We narrowed to 99,974 results for: %s
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Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Con/Fon-NpHR3.3-EYFP
Plasmid#137152PurposeIntersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND FlpDepositorHas serviceAAV8InsertCon/Fon-NpHR3.3-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-nEF-Con/Fon-NpHR3.3-EYFP
Plasmid#137152PurposeIntersectional viral expression of NpHR3.3-EYFP in cells expressing both Cre AND FlpDepositorHas serviceAAV8InsertCon/Fon-NpHR3.3-EYFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationW179FPromoternEFAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-Flex-dTomato-Fishell_7
Plasmid#83894PurposeCre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV Retrograde, AAV1, AAV2, AAV5, and AAV9InserttdTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-Flex-dTomato-Fishell_7
Plasmid#83894PurposeCre recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV Retrograde, AAV1, AAV2, AAV5, and AAV9InserttdTomato
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXL002-ishRNA-beta-catenin-1
Plasmid#36297DepositorInsertshRNA of beta-catenin-1 (CTNNB1 Human)
UseLentiviral and RNAiTagsIRES-RFP-PuroExpressionMammalianPromoterTetO-H1Available sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE
Plasmid#179459PurposeDouble floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorHas serviceAAV1InsertJEDI-2P-Kv
UseAAV and Cre/LoxExpressionMammalianPromoterEF1aAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRetroX GFP T2A Cre
Plasmid#63704PurposeTetracycline or doxycycline inducible expression of GFP T2A Cre fusion in mammalian cellsDepositorInsertT2A Cre
UseRetroviralTagsEGFPExpressionMammalianPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209722PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.HaloTag
UseAAVExpressionMammalianPromoterCAGAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCJ190
Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRExpressionMammalianPromoterPgkAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LifeAct-mGreenLantern
Plasmid#164459PurposeLocalization of mGreenLantern fluorescent protein to actinDepositorInsertmGreenLantern
TagsLifeActExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMGF182
Plasmid#97006PurposeExpresses GFP-CHMP7 in mammalian cells under a crippled promoterDepositorAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneTagsHAExpressionMammalianAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cofilin1_pLVX-EF1-Cry2-mCherry
Plasmid#64370Purposelentiviral expression of Cofilin1 and Cry2-mCherryDepositorAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-mCherry-cNLS
Plasmid#138527PurposeInducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
Tags3x SV40 cNLSExpressionMammalianPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHmut
Plasmid#131637PurposeExpresses APOBEC1 fused to a mutant YTH domain with diminished m6A bindingDepositorInsertAPOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
TagsHAExpressionMammalianMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MS2_SC2_S_Delta
Plasmid#175950PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsExpressionBacterialMutationDelta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL2- Empty control -SV40-Luc
Plasmid#26280PurposeAddgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR-3xFLAG-YAP1-S127A
Plasmid#42239DepositorInsertYAP1 (YAP1 Human)
UseGateway CloningTags3xFLAGMutationSerine 127 changed to alaninePromoternoneAvailable sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by