We narrowed to 150,751 results for: ins

1. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-LoxP-GFP-LoxP-SNAP-TERT

   Plasmid

   #71391

   Purpose

   Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Use

   Hr donor

   Tags

   FLAG-SNAP

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CD-MPR-mCherry-RUSH

   Plasmid

   #202798

   Purpose

   RUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPR

   Depositor

   Juan Bonifacino

   Article

   Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

   Insert

   M6PR (M6PR Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed 40 K (AAA) with R (AGA)

   Promoter

   CMV

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTRE-BI-TRIM21

   Plasmid

   #207838

   Purpose

   Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   TRIM21 (TRIM21 Human)
   mCherry
   hIgG1-FC
   VHH-GFP4
   

   Tags

   weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX_TRC311 ETV4-L

   Plasmid

   #74982

   Purpose

   Constitutive ETV4 ORF, blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ETV4 (ETV4 Human)
   

   Expression

   Mammalian

   Mutation

   S396N

   Promoter

   EF1a

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5FRT_KCNQ3

   Plasmid

   #173163

   Purpose

   To generate Flp-In CHO-KCNQ3 stable cells

   Depositor

   Al George

   Article

   Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

   Insert

   KCNQ3 (KCNQ3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. R2pH-LAMPx3ALFA

   Plasmid

   #225136

   Purpose

   R2pH-LAMP1-labelled lysosomes with 3xLFA

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTriEx-mVenus-PA-Rac1

    Plasmid

    #22007

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

    Plasmid

    #114112

    Purpose

    fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    NanoLuc; Volvox channelrhodopsin 1; EYFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-C1 RPEL1-EGFP-3XNLS

    Plasmid

    #58469

    Purpose

    Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    MRTF-A RPEL1 (Mrtfa Synthetic, Mouse)
    3X NLS with 3X stop codons
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Gene Trap - 24XMS2 Lentiviral Vector

    Plasmid

    #174198

    Purpose

    Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

    Depositor

    Daniel Larson

    Article

    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

    Insert

    iRFP
    

    Use

    Lentiviral

    Tags

    24X MS2 hairpins, Blasticidin, and T2A

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLX_TRC311 ETV4-S

    Plasmid

    #74983

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 )
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFB-EPCP5

    Plasmid

    #228879

    Purpose

    Expression of Z-tagged LRRK2 KW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1846

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-Foxo1-ADA 6KR

    Plasmid

    #17561

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1-ADA 6KR (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

20. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge