We narrowed to 9,499 results for: pho;

1. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

   Plasmid

   #107726

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GRB2-SHC

   Plasmid

   #139623

   Purpose

   StrepIII-HA-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

   Insert

   GRB2 (GRB2 Human)
   

   Tags

   StrepIII-HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MIGR1-cyclin E-AA

   Plasmid

   #47498

   Purpose

   MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

   Depositor

   Alex Minella

   Article

   Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

   Insert

   cyclin E1 (CCNE1 Human)
   

   Use

   Retroviral; Mscv-ires-gfp

   Expression

   Mammalian

   Mutation

   threonines 62 and 380 to alanine (T62A T380A)

   Promoter

   LTR

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pBT3037

   Plasmid

   #122544

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Synthetic, Nematode)
   

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-HE-Glyco-Venus-bPAC(S27A)

   Plasmid

   #165490

   Purpose

   Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Glyco-Venus-bPAC(S27A)-Myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry-Zdk1-RhoA Q63L

   Plasmid

   #81058

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

   Plasmid

   #99229

   Purpose

   Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos2
   

   Tags

   Lifeact

   Expression

   Mammalian

   Mutation

   A69T for primed conversion applications

   Promoter

   CMV

   Available Since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-mCherry-Zdk1-Rac1 Q61L

   Plasmid

   #81059

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 Q61L (RHOA Human)
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pUC18T-mini-Tn7T-Gm-Pc-mKate2

   Plasmid

   #199249

   Purpose

   Fluorescent labelling of bacterial cells with mKate2

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized mKate2 gene
   

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-mVenus-Zdk1-RhoA Q63L

    Plasmid

    #81046

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dox-Akt

    Plasmid

    #207328

    Purpose

    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

    Depositor

    Jared Toettcher

    Article

    Underhill et al Development. 2023 Aug 15;150(16):dev2016

    Insert

    rac protein kinase-alpha (AKT1 Human)
    

    Tags

    myristoylation tag - BFP

    Expression

    Bacterial

    Mutation

    range: 129 - 480

    Promoter

    Tet Response Element

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-FLAG-GPD1L

    Plasmid

    #32486

    Depositor

    Pere Puigserver

    Article

    Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

    Insert

    GPD1L (Gpd1l Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pOP22-5'UTR-BBa_B0034

    Plasmid

    #216626

    Purpose

    NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

    Depositor

    Henrike Niederholtmeyer

    Article

    Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

    Insert

    T7p-5'B0034-NanoLuc-3'TMV
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid

    #141236

    Purpose

    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    NES-jRGECO1a
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    6xHIS tag and nuclear export signal

    Promoter

    human elongation factor-1 alpha (EF-1 alpha)

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-Zdk1-Rac1 Q61L

    Plasmid

    #81137

    Purpose

    mammalian expression of Zdk1 tagged Rac1 Q61L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    Rac1 (RHOA Human)
    

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. hMLKL-Venus-Mito

    Plasmid

    #106080

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    hMLKL-Venus-Mito (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    mitochondrial-targetting domain of Bcl-xl

    Expression

    Mammalian

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3−3xFLAG−AGO2(S387D)

    Plasmid

    #220463

    Purpose

    Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

    Depositor

    David Bartel

    Article

    Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

    Insert

    Human AGO2 (AGO2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Serine 387 to aspartate

    Promoter

    CMV

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SARS_COV2_N_HA_BASU

    Plasmid

    #153989

    Purpose

    Express N gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-mScarlet-gamma9

    Plasmid

    #140097

    Purpose

    Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    GNGT2
    

    Tags

    N-terminal mScarlet tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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