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pENTR-p27(kip1)

Plasmid

#50026

Purpose

pENTR vector for human cyclin-dependent kinase inhibitor 1B

Depositor

Jamie Davies

Article

Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

Insert

p27(kip1) (CDKN1B Human)

Use

Entry vector

Available Since

Dec. 10, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00606

Plasmid

#39684

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG15715 (CG15715 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC3

Plasmid

#72712

Purpose

Lentiviral expression of shRNA against CRTC3 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC3

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
p-mCherry2-sgC9cen

Plasmid

#198326

Purpose

Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

Chr9-CEN guide RNA

Expression

Mammalian

Promoter

hU6

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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KP201: pMVP (L5-L4) eGFP-P2A

Plasmid

#121708

Purpose

pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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KP301: pMVP (L5-L4) mCherry-P2A

Plasmid

#121710

Purpose

pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

mCherry-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB526

Plasmid

#74868

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLY239

Plasmid

#184152

Purpose

Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-R3

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLY273

Plasmid

#184155

Purpose

Reprogrammed short tracrRNA for hijacking mRNA of zraP

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

s-tracrRNA-zraP-22

Use

Synthetic Biology

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KHBD00433

Plasmid

#34538

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9745 (D1 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
HE401: pMVP (L1-R5) shRNA

Plasmid

#121698

Purpose

pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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sspa-GCaMP6f

Plasmid

#67561

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159860

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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LF601: pMVP (L1-R5) TBG promoter

Plasmid

#121695

Purpose

pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6f

Plasmid

#67558

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pSR353

Plasmid

#125078

Purpose

Expression of narL under PLtetO-1, output promoter PdcuS77

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL (narL )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB427

Plasmid

#74821

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB426

Plasmid

#74820

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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