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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 7061 - 7080 of 16144 results

  1. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid
    #198552
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198552-plasmid-map-sequence-id-388895

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  6. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  12. pYPQ230-RVR

    Plasmid
    #108861
    Purpose
    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations
    Depositor
    Yiping Qi
    Article
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R, K554V and Y558R, Cpf1 is rice codon optimi…
    Available Since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    108861-plasmid-map-sequence-id-209929

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  14. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid
    #208967
    Purpose
    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208967-plasmid-map-sequence-id-415814

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  20. pDTFendoNA2

    Plasmid
    #174731
    Purpose
    Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386F
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174731-plasmid-map-sequence-id-343522

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Showing: 7061 - 7080 of 16144 results