We narrowed to 15,981 results for: LEA

1. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJWK_VD_02

   Plasmid

   #158103

   Purpose

   5'UTR stem-loop luciferase reporter: -30kcal/mol

   Depositor

   Ted Dawson

   Article

   Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

   Insert

   5'UTR synthetic stem-loop -30kcal/mol
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-mM₃O-IP

   Plasmid

   #46644

   Depositor

   Nobuaki Kikyo

   Article

   Hirai et al Stem Cells. 2011 Sep;29(9):1349-61. doi:

   Insert

   mM₃O (Pou5f1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES Puro and mouse MyoD (aa 1-62)

   Expression

   Mammalian

   Mutation

   contains a fragment of mouse MyoD (aa1-62) fused …

   Available Since

   July 22, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pLR5-CAG-NLS-tdiRFP670-2A-LBR

   Plasmid

   #82620

   Purpose

   Expresses NLS-tdiRFP-2A-LBR in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)
   

   Use

   Piggybac vector

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVL.SSA TLR (CCR5 TALEN Sce Spacer)

   Plasmid

   #46941

   Purpose

   Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with TALEN TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   embedded TALEN TS from 163-218, truncated 25 amin…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pαH-S-HRV3C/D614G

   Plasmid

   #164568

   Purpose

   SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-HRV3C-D614G variant)
   

   Tags

   2X Strep-Tag II and 8X His tag

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPRIME-CMV-GFP-shDyn2

   Plasmid

   #132712

   Purpose

   Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

   Depositor

   Robert Messing

   Article

   Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

   Insert

   shDyn(2) (Pdyn Rat)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_RBMX-eA3A-UdgX-NG-nCas9

   Plasmid

   #163551

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKO_Ptbp_ex8_3

    Plasmid

    #155083

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_HA.S.VSVg_mCherry-NLS

    Plasmid

    #178269

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HA.HSV.VSVg_mCherry-NLS

    Plasmid

    #178261

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_HA.HSV.S_mCherry-NLS

    Plasmid

    #178260

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    HA.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_C.S.VSVg_mCherry-NLS

    Plasmid

    #178255

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.S.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_C.HSV.VSVg_mCherry-NLS

    Plasmid

    #178246

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_C.HSV.S_mCherry-NLS

    Plasmid

    #178245

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HSV.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_C.HA.VSVg_mCherry-NLS

    Plasmid

    #178241

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_C.HA.S_mCherry-NLS

    Plasmid

    #178240

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.S and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_C.HA.HSV_mCherry-NLS

    Plasmid

    #178237

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    C.HA.HSV and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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