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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,360 results for: Tat

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Showing: 7061 - 7080 of 19360 results

  2. pKLV-EF1aGFP2AKAT7-linker-mAID-W

    Plasmid
    #159294
    Purpose
    Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, mAID-tagged) driven by human EF1a promoter
    Depositor
    Kosuke Yusa
    Article
    Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:
    Insert
    KAT7 (KAT7 Human)
    Use
    Lentiviral
    Tags
    mAID (auxin-inducible degron)-tagged
    Expression
    Mammalian
    Mutation
    CRISPR sites mutated (gRNA ID. 5 and A10)
    Promoter
    human EF1a promoter
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    159294-plasmid-map-sequence-id-313584

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  3. B-SpCas9-HF1

    Plasmid
    #126762
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-SpCas9-HF1 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than SpCas9HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-SpCas9-HF1
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; Q926A; amino acids 1005-1013…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126762-plasmid-map-sequence-id-410965

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  6. pLL3.7-EF-EYFP-YAP1_delta5C-PolyA

    Plasmid
    #112289
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
    Depositor
    Erik Sahai
    Article
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma lacking the last 5 Amino acids …
    Promoter
    EF-1 alpha
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    112289-plasmid-map-sequence-id-213521

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  9. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV-F
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

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  10. pBS7

    Plasmid
    #83774
    Purpose
    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    Venus
    Tags
    Venus
    Expression
    Yeast
    Mutation
    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …
    Available Since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    83774-plasmid-map-sequence-id-159984

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  14. JARID1BA

    Plasmid
    #74648
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JARID1BA (KDM5B Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    Leu101-(Gly)4-Ala374 deletion mutant
    Promoter
    PH
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. RBP2-like-bio

    Plasmid
    #68536
    Purpose
    Expresses full-length P. vivax RBP2-like (PVX_101590) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Depositor
    Julian Rayner, Gavin Wright
    Article
    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0
    Insert
    RBP2-like
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    68536-plasmid-map-sequence-id-120662

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Showing: 7061 - 7080 of 19360 results