We narrowed to 166,390 results for: addgene

1. ADAMTS1_pCSdest

   Plasmid

   #53807

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ADAMTS1 (ADAMTS1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pAC93-pmax-dCas9VP160

   Plasmid

   #48225

   Purpose

   dCas9VP160 on pmax expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR

   Tags

   HA Tag and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova)

   Plasmid

   #85041

   Purpose

   This plasmid should be used for Cre/loxP-based Supernova-CRISPR/Cas9.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   loxP-stop-loxP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPS0382

   Plasmid

   #133232

   Purpose

   Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

   Depositor

   Philip Poole

   Article

   Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Insert

   constructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. DSCAM_pCSdest

   Plasmid

   #53906

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   DSCAM (DSCAM Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP - FLAG-APEX2- hs Lamin B1

   Plasmid

   #139442

   Purpose

   APEX2-Lamin B1 expression plasmid

   Depositor

   Yixian Zheng

   Article

   Tran et al bioRxiv 2020.02.05.935635

   Insert

   LMNB1 (LMNB1 Human)
   

   Tags

   3xGGGGS linker (between APEX2 and Lamin B1), APEX…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV-IFP2.0 T2A HO1

   Plasmid

   #59427

   Purpose

   Express infrared fluorescent protein IFP2.0 plus HO1 enzyme

   Depositor

   Xiaokun Shu

   Article

   Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

   Insert

   IFP2.0T2AHO1 IRES EGFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

   Plasmid

   #129668

   Purpose

   A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKC014

    Plasmid

    #226623

    Purpose

    pLenti wt hTdT template for library 3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP775_αGCN4-p65HSF1-CO

    Plasmid

    #211768

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-p65HSF1
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV PIG Erg

    Plasmid

    #66984

    Purpose

    Retroviral expression plasmid for mouse Erg expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (Erg Mouse)
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-GAL4-iLight-VP16

    Plasmid

    #170274

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
    

    Use

    AAV

    Mutation

    See Comments

    Promoter

    CaMKII

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

    Plasmid

    #134337

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Efa
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-CAG-oScarlet-WPRE-BC5-pA

    Plasmid

    #220769

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC5
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV-CAG-oScarlet-WPRE-BC4-pA

    Plasmid

    #220768

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC4
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-CAG-oScarlet-WPRE-BC3-pA

    Plasmid

    #220767

    Purpose

    Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

    Depositor

    Vijay Samineni

    Article

    Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

    Insert

    oScarlert-WPRE-BC3
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hsyn-GRAB_HA1m

    Plasmid

    #190474

    Purpose

    Express the medium-affinity histamine sensor GRAB_HA1m in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1m (medium affinity)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX335-EN475

    Plasmid

    #86231

    Purpose

    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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