We narrowed to 166,390 results for: addgene

1. LLP778_Traptavidin-p65HSF1-CO

   Plasmid

   #211771

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-p65HSF1
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP776_SpyCatcher-p65HSF1-CO

   Plasmid

   #211769

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-p65HSF1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ERG_pCSdest

   Plasmid

   #53864

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ERG (ERG Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

4. MAC3-FGFR1

   Plasmid

   #222611

   Purpose

   Expression of MAC3-tagged FGFR1

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   FGFR1 (FGFR1 Human)
   

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

   Plasmid

   #134340

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIC1
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OA-1053A

   Plasmid

   #184006

   Purpose

   Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   U6b:sgRNAeve1 - U6c:sgRNAeve2
   

   Expression

   Insect

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHC039 (∆phcA)

   Plasmid

   #226207

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for PhcA
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

   Plasmid

   #199328

   Purpose

   mutated RHIM motive (interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed V458, Q459,V460 and G461 to alanine

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMW96

    Plasmid

    #178066

    Purpose

    Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

    Insert

    CDK5RAP2 (CDK5RAP2 Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAC153-dual-dCas9VP96-sgExpression

    Plasmid

    #48239

    Purpose

    Dual expression construct expressing both dCas9VP96 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP96

    Expression

    Mammalian

    Mutation

    D10A H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    U6 upstream enhancer sequence

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

    Plasmid

    #225943

    Purpose

    Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid

    #83107

    Purpose

    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

    Depositor

    Andrew Brooks

    Inserts

    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    

    Use

    Lentiviral

    Tags

    2A self cleaving peptide

    Expression

    Mammalian

    Mutation

    R24C and p64 isoform

    Promoter

    hPGK and hPGK (single transcript)

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUT_mNF-Cas13d-Triplex

    Plasmid

    #227709

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d-Triplex
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV-d2i

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBabe HA-Runx1-neo

    Plasmid

    #45815

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

17. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LLP617_αGCN4-mCherry

    Plasmid

    #211785

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SnoopCatcher-DNMT3A
    

    Tags

    2xOLLAS

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LLP783_SpyCatcher-DNMT3A-CO

    Plasmid

    #211776

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavudin-DNMT3A
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge