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pDSG318

Plasmid

#115595

Purpose

surface-displayed Ag2, used in composability experiments; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NINJ1

Plasmid

#201180

Purpose

Transient expression of NINJ1 gene in mammalian cells

Depositor

Jonathan Kagan

Article

Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

Insert

Ninjurin 1 (Ninj1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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CSII-prEF1a-E2-Crimson-NLS

Plasmid

#125263

Purpose

Fluorescent reporter for cell size

Depositor

Jan Skotheim

Article

Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

Insert

E2-Crimson-NLS

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_ELAVL1

Plasmid

#106104

Purpose

Inducible gene expression of FlagHA ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

ELAVL1 (ELAVL1 Human)

Tags

Flag and HA

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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P628_SpCas9-RecA

Plasmid

#87263

Purpose

Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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N1-mSin1.2-GFP

Plasmid

#72908

Purpose

GFP-tagged mSin1 (isoform 2)

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Sox2

Plasmid

#26351

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGL3-EF1a-i53

Plasmid

#216243

Purpose

Express i53 in mammalian cells as a HDR enhancer

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

i53

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Cherry-ICP0-C3

Plasmid

#134560

Purpose

Expresses Cherry-ICP0 fusion protein

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-GLIS1 (FLJ33315SAAN)

Plasmid

#33274

Purpose

Retroviral expression of canonical human GLIS1

Depositor

Shinya Yamanaka

Article

Maekawa et al Nature. 2011 . 474(7350):225-9.

Insert

GLIS1, Gateway casette A (GLIS1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pLSG (stra8)

Plasmid

#21619

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Stra8 promoter (Stra8 Mouse)

Use

Lentiviral

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pSH1/M-FGFR3-Fv-Fvls-E

Plasmid

#15287

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR3 cytoplasmic domain only FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pWZL Blast H-Ras G12V T35S

Plasmid

#12278

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V Y40C

Plasmid

#12279

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V Y40C (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The Y40C mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pMK425 (GAL-OsTIR1(F74A))

Plasmid

#140657

Purpose

GAL-OsTIR1(F74A)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74A)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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