We narrowed to 8,732 results for: Set

1. pgRNA_hMECP2_promoter_No4

   Plasmid

   #194899

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-4 targeting the promoter of human MECP2

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC70

   Plasmid

   #118878

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, cleavable with TEV protease; HIS6, cleavabl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA4_MYOD1

   Plasmid

   #64139

   Purpose

   Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_MYOD1 (MYOD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC032 - RcdCas13a from Rhodobacter capsulatus DE442

   Plasmid

   #91922

   Purpose

   Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcdCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

   Plasmid

   #91920

   Purpose

   Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcsCas13a and guide expression cassette
   

   Expression

   Bacterial

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. T7-His-GFP-Arp11

   Plasmid

   #51402

   Purpose

   expression of GFP-tagged mouse Arp11 under T7 promoter

   Depositor

   Trina Schroer

   Article

   Eckley et al Mol Biol Cell. 2003 Jul;14(7):2645-54. E

   Insert

   Arp11 (Actr10 Mouse)
   

   Tags

   GFP and His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCALNL-eGFP-NT1-6-gix-6-NT1

   Plasmid

   #81204

   Purpose

   contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Expression

   Mammalian

   Promoter

   pCBa

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2353_Tier3(PB)-YPet_PuroR

   Plasmid

   #169652

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC_mNG2(11)_BSDminus_F1

   Plasmid

   #184163

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mNG2(11)
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179077

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)
    

    Expression

    Yeast

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

    Plasmid

    #91913

    Purpose

    Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    CaCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-myr-eGFP-SV40pA-FKF

    Plasmid

    #119866

    Purpose

    Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSwith-tdTomato

    Plasmid

    #60953

    Purpose

    Cre recombinase-mediated cassette exchange

    Depositor

    Liangxue Lai

    Article

    Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

    Insert

    tdTomato
    

    Use

    Cre/Lox

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLyGo-Ec-6

    Plasmid

    #163132

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLib6.4B-Aaeg_774

    Plasmid

    #176673

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLib6.4B-Cquin_596

    Plasmid

    #176669

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA3_MYOD1

    Plasmid

    #64138

    Purpose

    Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_MYOD1 (MYOD1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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