We narrowed to 14,567 results for: cas9 genes

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gfap-EGFP donor

Plasmid

#65564

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap-EGFP (gfap Zebrafish)

Use

Zebrafish injection

Promoter

none

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pX330-COSMC-KO

Plasmid

#80008

Purpose

gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

COSMC (C1GALT1C1 Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-MGAT1-KO

Plasmid

#80009

Purpose

gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

Depositor

Sriram Neelamegham

Article

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

MGAT1 (MGAT1 Human)

Use

CRISPR

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-GFP-LoxP-SNAP-TERT

Plasmid

#71391

Purpose

Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.

Depositor

Thomas Cech

Article

Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

Insert

TERT (TERT Human)

Use

Hr donor

Tags

FLAG-SNAP

Promoter

Endogenous TERT promotor

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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px330 Rosa26 Gu1 (LH416)

Plasmid

#99629

Purpose

Plasmid used to target the wildtype Rosa26 allele with Cas9

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Cas9

Use

CRISPR

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-mitf

Plasmid

#69801

Purpose

Used to make mitf knockout porcine cell line

Depositor

Jianguo Zhao

Article

Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

Inserts

mitf-sgRNA
SpCas9

Expression

Mammalian

Promoter

Chicken Beta-Actin and U6

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

Plasmid

#160627

Purpose

TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-MS2:TV-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

Plasmid

#160571

Purpose

Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNAscaffold 2.1 scRNA

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

Plasmid

#160622

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s_MS2:EDLL_Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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PV225

Plasmid

#132334

Purpose

dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))

Tags

AT-CBF1

Expression

Plant

Mutation

Codon optimize for expression and stability in Ze…

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168242

Purpose

"Rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-WT

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-Exon3

Plasmid

#209075

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-Exon3 HDRT (PRF1 Human)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-T2A-cDNA

Plasmid

#209076

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-T2A-cDNA HDRT (PRF1 Human)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

Plasmid

#160609

Purpose

TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2-VP64:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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