We narrowed to 4,352 results for: GAI

1. pT2-7xTcf-NLS-ONL

   Plasmid

   #65713

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-Foxo1-WT 6KR

   Plasmid

   #17558

   Depositor

   Domenico Accili

   Article

   Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

   Insert

   Foxo1 6KR (Foxo1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K242R, K246R, K259R, K262R, K271R, K291R

   Promoter

   CMV

   Available since

   Oct. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgFFL

   Plasmid

   #59877

   Purpose

   synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene.

   Depositor

   Georg Seelig

   Article

   Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.

   Insert

   mCherry with intron containing the mouse mir-124–3
   

   Use

   Tags

   PEST and VAMP 3 UTR

   Expression

   Mammalian

   Mutation

   Promoter

   doxycycline (Dox)-inducible promoter (CMV/TO)

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS540-pCSDest2-AcrIIC4Hpa

   Plasmid

   #113434

   Purpose

   Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS542-pCSDest2-AcrIIC5Smu

   Plasmid

   #113435

   Purpose

   Expresses type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVL94 - pOp4:VND7

   Plasmid

   #116016

   Purpose

   destination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-induction

   Depositor

   Thomas Greb

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Scara3-IRES-BFP

   Plasmid

   #117265

   Purpose

   Retroviral overexpression of Scara3

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Insert

   Scara3 (Scara3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   LTR

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3myc-PR-LBD

   Plasmid

   #89113

   Purpose

   mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

   Depositor

   Elizabeth Wilson

   Article

   Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

   Insert

   human progesterone receptor ligand binding domain (PGR Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains Ligand Binding Domain (632-933)

   Promoter

   CMV

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pVL90 - VND7

    Plasmid

    #116035

    Purpose

    entry vector for GreenGate cloning method, CDS

    Depositor

    Thomas Greb

    Article

    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

    Insert

    VND7 (VND7 Mustard Weed)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM-ErbB4CTF-PY3 mutant

    Plasmid

    #17799

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pM-ErbB4-delta-kinase

    Plasmid

    #17800

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 988-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

13. p5M-PR-B-1-688

    Plasmid

    #89115

    Purpose

    mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

    Insert

    human progesterone receptor-B N-term+DNA binding domain (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 restriction sites deleted in polylinker, contai…

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mlp-hCHES1sh

    Plasmid

    #51845

    Purpose

    shRNA against CHES1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    shRNA against CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pM-ErbB4CTF-PY1 mutant

    Plasmid

    #17798

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Use

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292, …

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pTone-gata2aECE-EGFP

    Plasmid

    #132974

    Purpose

    gata2a endothelial enhancer (x6) and basal promoter driving egfp in pTol1 backbone

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Inserts

    eGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    

    Use

    Unspecified; Transposon-mediated

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-R-GECO1

    Plasmid

    #32444

    Purpose

    Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. PUC57_BAG3wt-eGFP

    Plasmid

    #98182

    Purpose

    Codes for full length, GFP tagged, human wildtype Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3wt-eGFP (BAG3 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB1.HMBP.PrS.PHF19

    Plasmid

    #125168

    Purpose

    Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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